Buile Dace, Pilmane Mara, Akota Ilze
Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradiņš University, 9 Kronvalda Str., LV-1010 Riga, Latvia.
Department of Maxillofacial Surgery, Institute of Stomatology, Riga Stradiņš University, 20 Dzirciema Str., LV-1007 Riga, Latvia.
Pediatr Rep. 2022 Oct 2;14(4):419-433. doi: 10.3390/pediatric14040050.
Cleft lip and palate (CLP) is one of the craniofacial defects. The objective of this study was to identify the differences in appearance between the tissue factors in cartilage of CLP patients after primary and secondary rhinoplasty. Immunohistochemistry was performed with MMP-2, MMP-8, MMP-9, TIMP-2, IL-1α, IL-10, bFGF, and TGFβ1. The quantification of the structures was performed using a semi-quantitative census method. MMP-2, -9, IL-1a, and bFGF demonstrated higher number of positive cells in patients, while the number of MMP-8, IL-1a, -10 and TGFβ1 cells was higher or equal in the control subjects. The only statistically significant difference between CLP-operated patients was found in the TIMP-2 group, where the primary CLP patient group had a higher number of TIMP-2 positive chondrocytes than the secondary CLP patient group (U = 53.5; = 0.021). The median value of the primary CLP group was ++ number of TIMP-2 positive chondrocytes compared to +++ in the secondary CLP group. No statistically significant difference was found between primary and secondary rhinoplasty patients for other tissue factors. Commonly, the rich expression of different tissue factors suggests a stimulation of higher elasticity in cleft affected cartilage. The statistically significant TIMP-2 elevation in primary operated cartilage indicates an impact of the selective tissue remodeling for hard tissue.
唇腭裂（CLP）是颅面缺陷之一。本研究的目的是确定唇腭裂患者一期和二期鼻整形术后软骨组织因子外观上的差异。采用免疫组织化学法检测基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-8（MMP-8）、基质金属蛋白酶-9（MMP-9）、金属蛋白酶组织抑制剂-2（TIMP-2）、白细胞介素-1α（IL-1α）、白细胞介素-10（IL-10）、碱性成纤维细胞生长因子（bFGF）和转化生长因子β1（TGFβ1）。使用半定量普查方法对结构进行量化。MMP-2、MMP-9、IL-1α和bFGF在患者中的阳性细胞数量较多，而MMP-8、IL-1α、IL-10和TGFβ1细胞数量在对照组中较高或相等。在CLP手术患者中，仅在TIMP-2组发现有统计学意义的差异，其中一期唇腭裂患者组的TIMP-2阳性软骨细胞数量高于二期唇腭裂患者组（U = 53.5；P = 0.021）。一期唇腭裂组TIMP-2阳性软骨细胞数量的中位数为++，而二期唇腭裂组为+++。对于其他组织因子，一期和二期鼻整形患者之间未发现统计学意义上的差异。通常，不同组织因子的丰富表达表明腭裂受累软骨的弹性更高受到了刺激。一期手术软骨中TIMP-2的统计学显著升高表明选择性组织重塑对硬组织有影响。
