School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Faculty of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China.
J Agric Food Chem. 2021 May 5;69(17):5134-5143. doi: 10.1021/acs.jafc.0c07658. Epub 2021 Apr 23.
Epicatechin gallate (ECG) is a main effective catechin widely existing in natural plants and food, with well-known health benefits. The present study first designed a new exosome-based delivery system for ECG and examined its neuroprotective effects on a rotenone (Rot)-induced Parkinson's disease (PD) model in vitro. Exosomes (Exo) were isolated from fresh bovine milk, and their average size was 85.15 ± 2.00 nm. ECG was encapsulated into Exo by a sonication method, and the loading efficiency of ECG-loaded exosomes (ECG-Exo) was 25.96 ± 0.45%. The neuroprotective effects of ECG-Exo were evaluated on Rot-induced SHSY5Y cells and compared with free ECG. Cell viability, cellular reactive oxygen species, apoptosis rate, and the expressions of caspase-3, Bax, Bcl-2, parkin, PINK1, and Atg5 were determined. Our results showed that Exo delivered ECG successfully into SHSY5Y cells and exhibited enhanced neuroprotective effects. ECG-Exo might inhibit SHSY5Y cell damage induced by Rot through antiapoptosis and antimitophagy.
没食子儿茶素没食子酸酯 (ECG) 是一种广泛存在于天然植物和食物中的主要有效儿茶素，具有良好的健康益处。本研究首次设计了一种基于外泌体的 ECG 新型递药系统，并在体外研究了其对鱼藤酮 (Rot) 诱导的帕金森病 (PD) 模型的神经保护作用。外泌体 (Exo) 从新鲜牛初乳中分离得到，平均粒径为 85.15±2.00nm。采用超声法将 ECG 包载于 Exo 中，ECG 载药外泌体 (ECG-Exo) 的载药效率为 25.96±0.45%。通过测定细胞活力、细胞内活性氧、细胞凋亡率以及 caspase-3、Bax、Bcl-2、parkin、PINK1 和 Atg5 的表达，评估了 ECG-Exo 对 Rot 诱导的 SHSY5Y 细胞的神经保护作用，并与游离 ECG 进行了比较。结果表明，Exo 成功地将 ECG 递送至 SHSY5Y 细胞，并表现出增强的神经保护作用。ECG-Exo 可能通过抗凋亡和抗自噬抑制 Rot 诱导的 SHSY5Y 细胞损伤。
