Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor, Malaysia.
Department of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor, Malaysia.
Biosci Biotechnol Biochem. 2021 Jun 24;85(7):1628-1638. doi: 10.1093/bbb/zbab072.
Juvenile hormone III (JH III) plays an important role in insect reproduction, development, and behavior. The second branch of JH III production includes oxidation of farnesol to farnesal by farnesol dehydrogenase. This study reported the identification and characterization of Plutella xylostella farnesol dehydrogenase (PxFoLDH). Our results showed that PxFoLDH belongs to the short-chain dehydrogenase/reductase superfamily, consisting of a single domain with a structurally conserved Rossman fold, an NAD(P) (H)-binding region and a structurally diverse C-terminal region. The purified enzyme displayed maximum activity at 55$\ $°C with pH 9.5 and was stable in the temperature below 70$\ ^\circ $C. PxFoLDH was determined to be a monomer with a relative molecular weight of 27 kDa and highly specific for trans, trans-farnesol, and NADP+. Among analog inhibitors tested, farnesyl acetate was the most effective inhibitor with the lowest Ki value of 0.02 µm. Our findings showed this purified enzyme may represent as NADP+-farnesol dehydrogenase.
保幼激素 III（JH III）在昆虫的繁殖、发育和行为中起着重要作用。JH III 生成的第二个分支包括法呢醇脱氢酶将法呢醇氧化为法呢醛。本研究报道了小菜蛾法呢醇脱氢酶（PxFoLDH）的鉴定和特性。我们的研究结果表明，PxFoLDH 属于短链脱氢酶/还原酶超家族，由一个具有结构保守的 Rossman 折叠的单一结构域、一个 NAD(P)（H）结合区和一个结构多样的 C 端区域组成。纯化后的酶在 55$\ $°C 时表现出最大活性，在 70$\ ^\circ $C 以下温度下稳定。PxFoLDH 被确定为一个单体，相对分子质量为 27 kDa，对反式，反式法呢醇和 NADP+具有高度特异性。在测试的类似物抑制剂中，法呢基乙酸酯是最有效的抑制剂，其最低 Ki 值为 0.02 µm。我们的研究结果表明，这种纯化的酶可能代表 NADP+-法呢醇脱氢酶。
