Lese Ioana, Tsai Catherine, Matter Martin, Wüthrich Tsering, Scheer Helene Sophie, Taddeo Adriano, Constantinescu Mihai Adrian, Herrmann Inge Katrin, Olariu Radu
Department of Plastic and Hand Surgery, Inselspital, Bern University Hospital, Freiburgstrasse 4, 3010 Bern, Switzerland.
Department for Biomedical Research, University of Bern, Murtenstrasse 50, 3008 Bern, Switzerland.
ACS Biomater Sci Eng. 2021 Jun 14;7(6):2676-2686. doi: 10.1021/acsbiomaterials.1c00283. Epub 2021 Apr 23.
Seroma formation is a well-recognized postoperative complication for many plastic and general surgical procedures. Although various tissue adhesives and substances have been used in an effort to treat seroma formation, no therapies have been established clinically. Recently, the nano-bridging phenomenon has been introduced as a promising approach to achieve tissue adhesion and strong closure of deep skin wounds in rats. The present study seeks to assess the potential of nano-bridging beyond skin wounds in a rat model of seroma. Seromas were induced in 20 Lewis rats through bilateral axillary lymphadenectomy, excision of the latissimus dorsi and cutaneous maximus muscles, and disruption of dermal lymphatics. On postoperative day (POD) 7, the seroma was aspirated on both sides. A bioactive nanoparticle (NP) suspension based on zinc-doped strontium-substituted bioglass/ceria nanoparticles (NP group) or fibrin glue (fibrin group) was injected into the right seroma cavity, while the left side was left untreated. On POD 14, the NP group showed complete remission (no seromas at all), while the fibrin group recorded a reduction of only 63% in the seroma fluid volume. The NPs exerted local anti-inflammatory and neo-angiogenic effects, without any detectable systemic changes. Moreover, the ceria levels recorded in the organs did not surpass the background level, indicating that the nanoparticles stayed at the site of application. This study is a promising first example demonstrating the ability of inorganic nanoparticle formulations to reduce seroma formation in a rat model, without any detectable systemic adverse effects. These results emphasize the potential of nanotechnological solutions in the therapeutic management of seroma in the clinical setting.
血清肿形成是许多整形手术和普通外科手术中公认的术后并发症。尽管已使用各种组织粘合剂和物质来治疗血清肿形成，但临床上尚未确立任何治疗方法。最近，纳米桥接现象作为一种有前景的方法被引入，用于在大鼠中实现组织粘连和深部皮肤伤口的牢固闭合。本研究旨在评估纳米桥接在大鼠血清肿模型中超出皮肤伤口的潜力。通过双侧腋窝淋巴结清扫、背阔肌和臀大肌切除以及真皮淋巴管破坏，在20只Lewis大鼠中诱导血清肿形成。术后第7天，双侧抽吸血清肿。将基于锌掺杂锶取代生物玻璃/二氧化铈纳米颗粒的生物活性纳米颗粒（NP）悬浮液（NP组）或纤维蛋白胶（纤维蛋白组）注入右侧血清肿腔，而左侧不进行处理。在术后第14天，NP组显示完全缓解（完全没有血清肿），而纤维蛋白组的血清肿液体积仅减少了63%。纳米颗粒发挥了局部抗炎和促血管生成作用，没有任何可检测到的全身变化。此外，在器官中记录的二氧化铈水平未超过背景水平，表明纳米颗粒停留在应用部位。这项研究是一个有前景的首个实例，证明了无机纳米颗粒制剂在大鼠模型中减少血清肿形成的能力，且没有任何可检测到的全身不良反应。这些结果强调了纳米技术解决方案在临床环境中血清肿治疗管理方面的潜力。
