Vidal P, Fernandez-Vigo J, Cabezas-Cerrato J
Chair of Physiopathology, Hospital General de Galicia Clinico Universitario, University of Santiago, Santiago de Compostela, Spain.
Acta Ophthalmol (Copenh). 1988 Apr;66(2):220-2. doi: 10.1111/j.1755-3768.1988.tb04017.x.
The extent of human cataract glycation has been determined by the 5-HMF/TBA method in 236 samples (53 from diabetic patients and 183 from non-diabetic controls). The brownish-yellow coloration typical of the advanced products of the Maillard reaction was measured spectrophotometrically. Glycation of crystallins was three times higher in diabetics than in controls, and was positively correlated with age in the latter group. The two groups did not differ significantly as regards the degree of browning, which was also uncorrelated with the primary products measured by the 5-HMF/TBA method. It is concluded that lens glycation is relatively mild and does not determine the intensity of the characteristic brownish-yellow colour of diabetic and senile cataracts. It is suggested that other coloured compounds produced with age interfere at the relevant wavelengths.
采用5-羟甲基糠醛/硫代巴比妥酸法测定了236份样本（53份来自糖尿病患者，183份来自非糖尿病对照）中人类白内障糖基化的程度。通过分光光度法测量了美拉德反应晚期产物典型的棕黄色。糖尿病患者晶状体的糖基化程度比对照组高3倍，且在对照组中与年龄呈正相关。两组在褐变程度上无显著差异，褐变程度也与5-羟甲基糠醛/硫代巴比妥酸法测定的初级产物无关。得出的结论是，晶状体糖基化相对较轻，并不决定糖尿病性和老年性白内障特征性棕黄色的强度。建议随着年龄增长产生的其他有色化合物在相关波长处产生干扰。
