Department of Immunology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano 1, Sección XVI, 14080, Tlalpan, Mexico City, Mexico.
Postgraduate Program in Health Science, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, Mexico.
Rheumatol Int. 2021 Jul;41(7):1329-1335. doi: 10.1007/s00296-021-04864-w. Epub 2021 Apr 23.
Monocytes play a key role in pathophysiology of antiphospholipid syndrome (APS), nevertheless it is unclear if microRNA expression is associated with particular APS features. Identify whether miR-19b-3p and miR-20a-5p expression in monocytes are associated with hallmarks of the APS. Fifty-seven APS patients and 18 healthy controls were studied. Expression of miR-19b-3p and miR-20a-5p was measured in monocytes by RT-qPCR. Both miR-19b-3p (AUC = 0.835, 95% CI 0.733-0.938; P < 0.001) and miR-20a-5p (AUC = 0.857, 0.757-0.957; P < 0.001) discriminated APS patients from healthy individuals. A cut-off point of 1.98 for miR-19-3p and 2.18 for miR-20a-5p showed that APS patients with low microRNA expression had higher levels of IgM and IgG anticardiolipin antibodies than patients with high microRNA expression. In addition, APS patients with low microRNA expression had higher IgG anti-β glycoprotein I antibody levels than their counterparts with high microRNA expression. Finally, miR-19b-3p and miR-20a-5p expression levels were significantly higher in APS patients using oral anticoagulants. Monocyte expression of miR-19b-3p and miR-20a-5p is low in APS, and patients with the lowest microRNA expression presented the highest levels of antiphospholipid antibodies.
单核细胞在抗磷脂综合征（APS）的病理生理学中发挥关键作用，但miRNA 表达是否与特定的 APS 特征相关尚不清楚。本研究旨在确定单核细胞中 miR-19b-3p 和 miR-20a-5p 的表达是否与 APS 的特征有关。研究纳入 57 例 APS 患者和 18 名健康对照者。采用 RT-qPCR 法检测单核细胞中 miR-19b-3p 和 miR-20a-5p 的表达。miR-19b-3p（AUC=0.835，95%CI 0.733-0.938；P<0.001）和 miR-20a-5p（AUC=0.857，0.757-0.957；P<0.001）均可区分 APS 患者和健康个体。miR-19-3p 的截断值为 1.98，miR-20a-5p 的截断值为 2.18，结果显示 miRNA 低表达的 APS 患者的 IgM 和 IgG 抗心磷脂抗体水平高于 miRNA 高表达的患者。此外，miR-19b-3p 和 miR-20a-5p 低表达的 APS 患者的 IgG 抗β糖蛋白 I 抗体水平高于 miRNA 高表达的患者。最后，使用口服抗凝剂的 APS 患者的 miR-19b-3p 和 miR-20a-5p 表达水平显著升高。APS 患者单核细胞中 miR-19b-3p 和 miR-20a-5p 的表达水平较低，miRNA 表达最低的患者抗磷脂抗体水平最高。
