Govaerts P J, Jacob W A, Marquet J
Department of ORL/ENT, University of Antwerp (U.I.A.), Antwerp-Wilrijk, Belgium.
Acta Otolaryngol. 1988 Mar-Apr;105(3-4):297-302. doi: 10.3109/00016488809097011.
A histological study was done on the thin, nearly transparent replacement membrane of tympanic membrane perforations. Human tympanic membranes that were rejected for transplantation, were studied by light and electron microscopy. The abrupt reduction in thickness at the margin of the covered perforation, is entirely due to the reduction of the lamina propria. Even in the thinnest parts of the replacement membrane, a lamina propria is present, separated by continuous basement membranes from the epithelium and mucosa, and measuring no more than some 2-3 microns in thickness. This lamina propria consists of fibrils and interfibrillar matrix, but fibroblasts appear to be lacking. The epithelial layer does not contain basal cells, confirming the thesis that the upper layers are not generated by in situ proliferation, but that they have migrated from the periphery.
对鼓膜穿孔处薄且近乎透明的替代膜进行了组织学研究。对因移植被拒收的人鼓膜进行了光镜和电镜研究。覆盖穿孔边缘处厚度的突然减小完全是由于固有层的减少。即使在替代膜最薄的部分，也存在固有层，它通过连续的基底膜与上皮和黏膜分隔开，厚度不超过约2 - 3微米。该固有层由纤维和纤维间基质组成，但似乎没有成纤维细胞。上皮层不含基底细胞，这证实了上层并非通过原位增殖产生，而是从周边迁移而来的观点。
