Stretchable and Transparent Electrochemical Sensor Based on Nanostructured Au on Carbon Nanotube Networks for Real-Time Analysis of HO Release from Cells.

Various electrochemical biosensors have been developed for direct and real-time recording of biomolecules released from living cells. However, since these traditional electrodes are commonly rigid and nonflexible, in situ monitoring of biochemical signals while cell deformation occurs remains a great challenge. Herein, we report a facile approach for the development of a stretchable and transparent electrochemical cell-sensing platform based on Au nanostructures (nano-Au) and carbon nanotube (CNT) films embedded in PDMS (nano-Au/CNTs/PDMS). The sandwich-like nanostructured network of nano-Au/CNTs endows the sensor with excellent mechanical stability and electrochemical performance. The obtained nano-Au/CNTs/PDMS electrode displays desired performance for HO detection with a wide linear range (20 nM-25.8 μM) and low detection limit (8 nM). Owing to good biocompatibility and flexibility, HeLa and human umbilical vein endothelial cells can be directly cultured on the electrode and real-time monitoring of HO release from cells under their stretched state was realized. The proposed strategy demonstrated in this work provides an effective way for design of stretchable sensors and more opportunities for sensing biomolecules from mechanically sensitive cells.