LDBIO Diagnostics, F-69009, Lyon, France.
Centre Hospitalier Universitaire de Bordeaux, Service de Parasitologie Mycologie, F-33000, Bordeaux, France.
Med Mycol. 2021 Sep 3;59(9):890-900. doi: 10.1093/mmy/myab018.
Food poisoning caused by toxic mushrooms, such as species in the Amanita genus, occurs frequently around the world. To properly treat these patients, it is important to rapidly and accurately identify the causal species. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry is a rapid technique that has been used in medical laboratories for the past three decades to identify bacteria, yeasts, and filamentous fungi.Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-Tof MS) is a rapid method used for the past three decades to identify microorganisms. In this study, we created and internally validated a MALDI-Tof MS reference database comprising 15 Amanita species frequently encountered in France, and we challenged this database with 38 Amanita specimens from four French locations, using a free online application for MALDI-ToF spectra identifications.Assessment of the database showed that mass spectra can be obtained by analyzing any portion of a carpophore and that all portions enabled identification of the carpophore at the species level. Most carpophores were correctly identified using our database, with the exception of specimens from the Vaginatae section. Decay tests also demonstrated that decayed portions (like those found in the kitchen garbage can) of Amanita phalloides mushrooms could be properly identified using MALDI-ToF MS.Our findings provide important insight for toxicology laboratories that often rely on DNA sequencing to identify meal leftovers implicated in food poisoning. In future developments, this technique could also be used to detect counterfeit mushrooms by including other genera in the reference database.
MALDI-ToF MS is a powerful identification tool for microorganisms. We demonstrate that the technique can be applied to Amanita specimens. This will prevent food intoxications as a rapid and definite identification can be obtained, and it can also be used for food remnants.
由毒蕈引起的食物中毒，如鹅膏菌属的某些物种，在世界各地经常发生。为了正确治疗这些患者，快速准确地识别致病物种非常重要。基质辅助激光解吸/电离飞行时间（MALDI-ToF）质谱分析是一种快速技术，在过去三十年中已在医学实验室中用于鉴定细菌、酵母和丝状真菌。基质辅助激光解吸电离飞行时间质谱分析（MALDI-ToF MS）是一种过去三十年中用于鉴定微生物的快速方法。在这项研究中，我们创建并内部验证了一个 MALDI-ToF MS 参考数据库，其中包含在法国经常遇到的 15 种鹅膏菌，并用来自法国四个地点的 38 个鹅膏菌标本对该数据库进行了挑战，使用了一个用于 MALDI-ToF 光谱鉴定的免费在线应用程序。对数据库的评估表明，可以通过分析子实体的任何部分来获得质谱，并且所有部分都可以在物种水平上鉴定子实体。使用我们的数据库可以正确识别大多数子实体，但 Vaginatae 部分的标本除外。衰减测试还表明，使用 MALDI-ToF MS 可以正确识别鹅膏菌子实体的衰减部分（如厨房垃圾桶中的部分）。我们的研究结果为毒理学实验室提供了重要的见解，这些实验室通常依赖 DNA 测序来识别与食物中毒有关的膳食残余物。在未来的发展中，通过在参考数据库中包含其他属，该技术还可以用于检测假冒蘑菇。
