Morais Francimauro S, Canuto Kirley M, Ribeiro Paulo R V, Silva Alison B, Pessoa Otilia D L, Freitas Cleverson D T, Bezerra Emanuel Alves, Gonçalves José F C, Souza Diego P, Sousa Brandon F, Silva Ayrles F B, Ramos Márcio V
Instituto Federal de Ciência e Tecnologia do Amazonas (IFAM), Itacoatiara, Amazonas 69109899, Brazil.
Embrapa Agroindústria Tropical, Fortaleza, Ceará, Brazil.
J Agric Food Chem. 2021 May 5;69(17):5049-5058. doi: 10.1021/acs.jafc.1c01177. Epub 2021 Apr 23.
is an important source of proteins and energy for humans and animals. However, postharvest losses caused by can reach from 20 to 100% of stored seeds. In this study, the insecticide potential of compounds extracted from latex was assessed. The latex was extracted with ethanol (70%) and then partitioned through sequential use of hexane and chloroform. These fractions were investigated by chromatography to determine their chemical composition. Plumieride, identified in a hydroalcoholic subfraction, was tested for insecticidal activity against . The ethanolic fraction (LC = 0.109; LC = 0.106%) and the plumieride (LC = 0.166; LC = 0.167%) were lethal to larvae. Plumieride (0.25%) delayed larval development, and mortality reached 100%. Its inhibitory action on intestinal α-amylase from larvae was higher (89.12%) than that of acarbose (63.82%). Plumieride (0.1%) inhibited the enzyme α-amylase in vivo in the larval intestine. This result was confirmed by a zymogram test performed by SDS-PAGE when the enzyme electrophoresed on gel copolymerized with starch. When spread on seeds, the hydroalcoholic fraction (1.0%) reduced infestation. The loss of seed mass was 5.26% compared to the control (44.97%). The results confirm the effect of latex compounds in protecting stored seeds against weevil infestation.
是人类和动物蛋白质及能量的重要来源。然而，由 造成的收获后损失可达储存种子的20%至100%。在本研究中，评估了从 乳胶中提取的化合物的杀虫潜力。用乙醇（70%）提取乳胶，然后依次用己烷和氯仿进行分配。通过色谱法研究这些馏分以确定其化学成分。在水醇亚馏分中鉴定出的羽扇豆醇，针对 测试其杀虫活性。乙醇馏分（LC = 0.109；LC = 0.106%）和羽扇豆醇（LC = 0.166；LC = 0.167%）对幼虫具有致死性。羽扇豆醇（0.25%）延缓幼虫发育，死亡率达100%。其对幼虫肠道α-淀粉酶的抑制作用（89.12%）高于阿卡波糖（63.82%）。羽扇豆醇（0.1%）在幼虫肠道内对α-淀粉酶具有体内抑制作用。当该酶在与淀粉共聚的凝胶上进行电泳时，通过SDS-PAGE进行的酶谱试验证实了这一结果。当将水醇馏分（1.0%）撒在种子上时，可减少虫害。与对照（44.97%）相比，种子质量损失为5.26%。结果证实了乳胶化合物在保护储存种子免受象鼻虫侵害方面的作用。
