Herrera-Calderón Oscar, Calero-Armijos Lisbeth Lucia, Cardona-G Wilson, Herrera-R Angie, Moreno Gustavo, Algarni Majed A, Alqarni Mohammed, El-Saber Batiha Gaber
Department of Pharmacology, Bromatology, Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, Peru.
Química de Plantas Colombianas, Faculty of Exact and Natural Sciences, Institute of Chemistry, University of Antioquia (UdeA), Calle 70 No. 52-21, Medellín 1226, Colombia.
Plants (Basel). 2021 Oct 16;10(10):2197. doi: 10.3390/plants10102197.
, also known as "Bellaco caspi", is a medicinal plant whose latex, stem bark, and leaves possess phenolic acids, lupeol, β-dihydro-plumbericinic acid, plumericin, and plumeride, among other components. Some of these have been linked to such biological activities as antiulcer, anti-inflammatory, and wound healing. The aim of this study was to determine the phytochemical compounds of latex, as well as its in vitro cytotoxicity and wound healing effect in mice. Latex was collected in the province of Iquitos, Peru. Phytochemical analysis was carried out with UPLC-ESI-MS/MS. The cytotoxicity was evaluated on two colon tumor cell lines (SW480 and SW620) and non-malignant cells (human keratinocytes, HaCaT, and Chinese hamster ovary, CHO-K1). The mice were distributed into two groups, as follows: Group I-control ( = 10; without treatment); II-( = 10) latex; wounds were induced with a scalpel in the dorsal-cervical area and treatments were applied topically twice a day on the incision for 10 days. Molecular docking was carried out on the glycogen synthase kinase 3β protein. Twenty-four chemical compounds were determined, mainly flavonoid-type compounds. Latex did not have a cytotoxic effect on tumor cells with IC values of more than 500 µg/mL. The latex had a regenerative effect on wounds in mice. Acacetin-7-O-neohesperidoside had the best docking score of -9.9 kcal/mol. In conclusion, latex had a wound healing effect in mice, as confirmed by histological study. However, a non-cytotoxic effect was observed on colon tumor cells SW480 and SW620.
，也被称为“Bellaco caspi”，是一种药用植物，其乳胶、茎皮和叶子含有酚酸、羽扇豆醇、β - 二氢 - 普卢米西尼克酸、普卢米辛和普卢米立德等成分。其中一些成分与抗溃疡、抗炎和伤口愈合等生物活性有关。本研究的目的是确定乳胶的植物化学成分，以及其在小鼠中的体外细胞毒性和伤口愈合效果。乳胶采集于秘鲁伊基托斯省。采用超高效液相色谱 - 电喷雾串联质谱法（UPLC - ESI - MS/MS）进行植物化学分析。对两种结肠肿瘤细胞系（SW480和SW620）和非恶性细胞（人角质形成细胞HaCaT和中国仓鼠卵巢细胞CHO - K1）进行细胞毒性评估。将小鼠分为两组，如下：第一组为对照组（ = 10；未治疗）；第二组（ = 10）为乳胶组；用手术刀在小鼠颈背部区域诱导伤口，并在切口处每天局部涂抹两次治疗药物，持续10天。对糖原合酶激酶3β蛋白进行分子对接。确定了24种化合物，主要是黄酮类化合物。乳胶对肿瘤细胞的IC值大于500 μg/mL时无细胞毒性作用。乳胶对小鼠伤口有再生作用。刺槐素 - 7 - O - 新橙皮糖苷的对接分数最佳，为 - 9.9 kcal/mol。总之，组织学研究证实，乳胶对小鼠有伤口愈合作用。然而，但对结肠肿瘤细胞SW480和SW620未观察到细胞毒性作用。
