Department of Biomedical Engineering, Research Center for Nano-Biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China; Shanxi Key Laboratory of Material Strength & Structural Impact, Institute of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Department of Biomedical Engineering, Research Center for Nano-Biomaterials & Regenerative Medicine, College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China.
Int J Biol Macromol. 2021 Jun 30;181:1039-1046. doi: 10.1016/j.ijbiomac.2021.04.114. Epub 2021 Apr 20.
Most existing hydrogel wound dressings lack gentle detachment property. In this work, novel hydrogels with anti-bacterial and induced detachment properties were prepared. Both gelatin (G) and sodium alginate (SA) are natural polymer materials. The G/SA hydrogels were prepared by dual cross-linking. The addition of SA significantly improves the mechanical properties of composite hydrogels. The tensile modulus and elongation at break of the G/SA hydrogels with 2.0% SA could reach 99.23 ± 2.18 kPa and 85.47 ± 5.01%, respectively. In addition, the interconnected porous network and high swelling ratio (over 9.99 ± 0.33) are beneficial to the transmission of oxygen and absorption of exudates to accelerate the healing of wound. Subsequently, berberine (BBR) was loaded into the G/SA hydrogels. The BBR/G/SA hydrogels show sustained drug release for 168 h and exhibit anti-bacterial effect against Staphylococcus aureus. The results of L929 cells cultured with the hydrogel extracts indicate good biocompatibility. Finally, results of EDTA-induced detachment performances demonstrate that the hydrogels could be removed from the wound as the internal structure destroyed. All illustrated results above demonstrated the BBR carried G/SA hydrogels have potential used as wound dressing materials in future.
大多数现有的水凝胶伤口敷料缺乏温和的脱离特性。在这项工作中，制备了具有抗菌和诱导脱离性能的新型水凝胶。明胶（G）和海藻酸钠（SA）都是天然聚合物材料。G/SA 水凝胶通过双重交联制备。SA 的加入显著提高了复合水凝胶的力学性能。SA 含量为 2.0%的 G/SA 水凝胶的拉伸模量和断裂伸长率分别可达 99.23 ± 2.18 kPa 和 85.47 ± 5.01%。此外，互穿的多孔网络和高溶胀比（超过 9.99 ± 0.33）有利于氧气的传递和渗出物的吸收，从而加速伤口的愈合。随后，将小檗碱（BBR）载入 G/SA 水凝胶中。BBR/G/SA 水凝胶表现出长达 168 小时的持续药物释放，并对金黄色葡萄球菌表现出抗菌作用。与水凝胶提取物共培养的 L929 细胞的结果表明其具有良好的生物相容性。最后，EDTA 诱导的脱离性能结果表明，水凝胶可以在内部结构破坏时从伤口上移除。所有这些结果都表明，负载 BBR 的 G/SA 水凝胶有望在未来用作伤口敷料材料。
