State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan, 430070, PR China.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, 430070, PR China; Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan, 430070, PR China.
Carbohydr Polym. 2018 Oct 1;197:292-304. doi: 10.1016/j.carbpol.2018.05.078. Epub 2018 May 26.
Hydrogels are widely used as debriding agents on account of providing a moist environment for wound healing, however the lack of mechanical strength, angiogenesis and antibacterial property limits their applications. In this study, we synthesized novel divalent ion cross-liking hydrogels (copper, zinc, strontium and calcium) and compared the mechanical performance, swelling ratio, antibacterial properties and biocompatibility in vitro and vivo. Thereinto, among the four divalent ions cross-linked hydrogels, copper ion crosslinking exhibited the maximum breaking strength, while strontium and zinc ion cross-linked hydrogels exhibited an excellent mechanical strength. In addition, the swelling ratio and pore size of no-ion cross-linked hydrogels was larger than ion cross-kinked hydrogels. In vitro, the improvements on wound healing after hydrogel application were evaluated by histological and molecular assays by detecting VEGF and TGF-β expression. In vitro and in vivo study results showed that zinc cross-kinked hydrogel had a spectrum of antibacterial activities, cell viability, mechanical strength and the ability of wound closure by promoting fibroblasts migration, vascularization, collagen deposition and the formation of granulation tissue.
水凝胶由于能为伤口愈合提供湿润的环境而被广泛用作清创剂，但由于缺乏机械强度、血管生成和抗菌性能，限制了其应用。在这项研究中，我们合成了新型二价离子交联水凝胶（铜、锌、锶和钙），并比较了它们在体外和体内的机械性能、溶胀比、抗菌性能和生物相容性。其中，在四种二价离子交联水凝胶中，铜离子交联的水凝胶具有最大的断裂强度，而锶和锌离子交联的水凝胶具有优异的机械强度。此外，无离子交联水凝胶的溶胀率和孔径大于离子交联水凝胶。通过检测 VEGF 和 TGF-β的表达，体外和体内实验评估了水凝胶应用后对伤口愈合的改善情况。体外和体内研究结果表明，锌交联水凝胶具有广谱的抗菌活性、细胞活力、机械强度和促进成纤维细胞迁移、血管生成、胶原沉积和肉芽组织形成的伤口闭合能力。
