Research Group on Development of Pharmaceutical Products (P&DProFar), Center for Biological and Health Sciences, Federal University of Western Bahia, Rua Bertioga, 892, Morada Nobre II, CEP 47810-059, Barreiras, BA, Brazil.
Laboratory of Immunopathology Keizo Asami, Department of Biochemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil.
J Ethnopharmacol. 2021 Jul 15;275:114100. doi: 10.1016/j.jep.2021.114100. Epub 2021 Apr 20.
The population has traditionally used the Mangifera indica plant leaves to treat diseases such as Diabetes Mellitus and alleviate signs and symptoms such as inflammation, diarrhea, and dysentery. In a previous study, we demonstrated that the flavonoids present in the aqueous extract from M. indica leaves (EAMI) exhibited a potent hypoglycemic effect in diabetic rats, promoting the widespread use of the plant by the population and highlighting the importance of investigating its oral toxicity.
The present study aimed to assess the toxic potential of EAMI in rats submitted to experimental models of acute and subacute (short-term) oral toxicity.
For the acute toxicity test, female Wistar rats received a single oral dose of 2000 mg/kg body weight of EAMI and were observed for 14 days. In the short-term toxicity test, male and female Wistar rats received repeated oral EAMI doses of 125, 250, 500 or 1000 mg/kg body weight and observed for 28 days.
The phytochemical analysis of EAMI demonstrated that the extract has high levels of flavonoids. No animals died in the acute toxicity test, and no clinical changes were observed that show signs of toxicity in the animals. There was no significant change in the weight of the organs of the animals submitted to tests with the EAMI, suggesting that LD is greater than 2000 mg/kg. In the conditions and doses tested in the short-term toxicity experiments, the treatment did not produce significant changes in the physiological, biochemical, hematological, and histopathological parameters in the animals evaluated.
Our study demonstrated that high doses of EAMI administered acutely, as well as all doses evaluated in the short-term oral toxicity model, should be considered safe during traditional therapeutic use.
该人群传统上使用芒果植物叶来治疗糖尿病等疾病，并缓解炎症、腹泻和痢疾等症状。在之前的一项研究中，我们证明了芒果叶水提物（EAMI）中存在的类黄酮在糖尿病大鼠中表现出很强的降血糖作用，这促进了该人群对该植物的广泛使用，并强调了调查其口服毒性的重要性。
本研究旨在评估 EAMI 在急性和亚急性（短期）口服毒性实验模型中大鼠的潜在毒性。
在急性毒性试验中，雌性 Wistar 大鼠接受单次口服 2000mg/kg 体重的 EAMI，并观察 14 天。在短期毒性试验中，雄性和雌性 Wistar 大鼠接受重复口服 EAMI 剂量为 125、250、500 或 1000mg/kg 体重，并观察 28 天。
EAMI 的植物化学分析表明，该提取物含有高水平的类黄酮。急性毒性试验中没有动物死亡，也没有观察到任何临床变化表明动物有毒性迹象。接受 EAMI 试验的动物器官重量没有显著变化，表明 LD 大于 2000mg/kg。在短期毒性试验中测试的条件和剂量下，该治疗未在评估的动物的生理、生化、血液学和组织病理学参数中产生显著变化。
我们的研究表明，急性给予高剂量的 EAMI 以及短期口服毒性模型中评估的所有剂量均应被认为在传统治疗用途中是安全的。
