Wang Shuting, Liu Huanliang, Qu Man, Wang Dayong
Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing 210009, China.
Key Laboratory of Environmental Medicine Engineering of Ministry of Education, Medical School, Southeast University, Nanjing 210009, China; Shenzhen Ruipuxun Academy for Stem Cell & Regenerative Medicine, Shenzhen 518122, China; College of Biology and Food Engineering, Chongqing Three Gorges University, Wanzhou 404100, China.
Ecotoxicol Environ Saf. 2021 Jul 1;217:112239. doi: 10.1016/j.ecoenv.2021.112239. Epub 2021 Apr 20.
Neurotransmission related signals are involved in the control of response to toxicants. We here focused on the tyramine and the glutamate related signals to determine their roles in regulating nanoplastic toxicity in Caenorhabditis elegans. In the range of μg/L, exposure to nanopolystyrene (100 nm) increased the expression of tdc-1 encoding a tyrosine decarboxylase required for synthesis of tyramine, and decreased the expression of eat-4 encoding a glutamate transporter. Both TDC-1 and EAT-4 could act in the neurons to regulate the nanopolystyrene toxicity. Meanwhile, neuronal RNAi knockdown of tdc-1 induced a susceptibility to nanopolystyrene toxicity, and neuronal RNAi knockdown of eat-4 induced a resistance to nanopolystyrene toxicity. In the neurons, TYRA-2 functioned as the corresponding receptor of tyramine and acted upstream of MPK-1 signaling to regulate the nanopolystyrene toxicity. Moreover, during the control of nanopolystyrene toxicity, GLR-4 and GLR-8 were identified as the corresponding glutamate receptors, and acted upstream of JNK-1 signaling and DBL-1 signaling, respectively. Our results demonstrated the crucial roles of tyramine and glutamate related signals in regulating the toxicity of nanoplastics in organisms.
神经传递相关信号参与了对毒物反应的控制。我们在此聚焦于酪胺和谷氨酸相关信号，以确定它们在调节秀丽隐杆线虫纳米塑料毒性中的作用。在微克/升范围内，暴露于纳米聚苯乙烯（100纳米）会增加编码酪胺合成所需酪氨酸脱羧酶的tdc-1的表达，并降低编码谷氨酸转运体的eat-4的表达。TDC-1和EAT-4均可在神经元中发挥作用，调节纳米聚苯乙烯的毒性。同时，tdc-1的神经元RNA干扰敲低会诱导对纳米聚苯乙烯毒性的易感性，而eat-4的神经元RNA干扰敲低会诱导对纳米聚苯乙烯毒性的抗性。在神经元中，TYRA-2作为酪胺的相应受体，在MPK-1信号传导上游发挥作用，调节纳米聚苯乙烯的毒性。此外，在纳米聚苯乙烯毒性控制过程中，GLR-4和GLR-8被鉴定为相应的谷氨酸受体，分别在JNK-1信号传导和DBL-1信号传导上游发挥作用。我们的结果证明了酪胺和谷氨酸相关信号在调节生物体中纳米塑料毒性方面的关键作用。
