Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba-PR 81531-980, Brazil.
Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Brazil, Centro Politécnico, CP 19011, Curitiba-PR 81531-980, Brazil.
Bioresour Technol. 2021 Aug;333:125174. doi: 10.1016/j.biortech.2021.125174. Epub 2021 Apr 16.
The use of low-cost feedstock for enzyme production is an environmental and economic solution. Sugarcane bagasse and soybean meal are employed in this study for optimised xylanase production with the concomitant synthesis of proteases. The enzymatic complex is produced by submerged fermentation by Aspergillus niger. Optimisation steps lead to a 2.16-fold increase in enzymatic activity. The fermentation kinetics are studied in Erlenmeyer flasks, a stirred tank reactor and a bubble column reactor, with the xylanase activities reaching 52.9; 33.7 and 60.5 U.mL, respectively. The protease production profile is also better in the bubble column reactor, exceeding 7 U.mL. The enzyme complex is then evaluated for the synthesis of xylooligosaccharides from sugarcane extracted xylan with a production of 3.1 g.L where xylotriose is the main product. Excellent perspectives are observed for the developed process with potential applications in the animal feed, prebiotics and paper industries.
利用低成本的原料生产酶是一种环境友好且经济的解决方案。本研究采用甘蔗渣和豆粕作为优化木聚糖酶生产的原料，同时合成蛋白酶。该酶复合物通过黑曲霉的深层发酵生产。优化步骤使酶活性提高了 2.16 倍。在摇瓶、搅拌釜反应器和鼓泡柱反应器中研究了发酵动力学，木聚糖酶活性分别达到 52.9、33.7 和 60.5 U.mL。在鼓泡柱反应器中，蛋白酶的产量也更好，超过 7 U.mL。然后，该酶复合物用于从甘蔗提取的木聚糖合成低聚木糖，产量为 3.1 g.L，其中木三糖是主要产物。该工艺具有广阔的应用前景，可应用于动物饲料、益生元和造纸等行业。
