Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Centro Politécnico, CP 19011, Curitiba-PR 81531-980, Brazil.
Bioprocess Engineering and Biotechnology Department, Federal University of Paraná, Centro Politécnico, CP 19011, Curitiba-PR 81531-980, Brazil.
Bioresour Technol. 2022 Oct;362:127800. doi: 10.1016/j.biortech.2022.127800. Epub 2022 Aug 23.
The application of biorefinery concepts to produce different value-added biomolecules such as xylooligosaccharides (XOs) generates economical competitive, sustainable and environmentally friendly processes. The objective of this work was to develop an efficient imidazole-pretreatment process of sugarcane bagasse (SB) and the use of the obtained hemicellulose fraction in the production of XOs with the application of in house produced xylanolytic enzymes using SB as substrate, under a biorefinery approach. SB imidazole pretreatment allowed the recovery of a hemicellulose rich fraction (34%) with 91.2% of delignification. Xylanase production by Aspergillus niger reached 53.1 U·mL at 120 h. The application of produced xylanases in the enzymatic hydrolysis of extracted xylan, allowed the production of 6.06 g·L of XOs, where xylotriose represented >70%. Great perspectives are viewed for the implementation of mixed processes in a sustainable closed cycle to produce biomolecules with concomitant valorization of subproducts from SB chain.
将生物炼制概念应用于生产不同附加值的生物分子，如木低聚糖 (XOs)，可以产生经济竞争力强、可持续且环保的工艺。本工作的目的是开发一种高效的甘蔗渣（SB）中咪唑预处理工艺，并在生物炼制方法下，利用内部生产的木聚糖酶从获得的半纤维素部分生产 XOs，以 SB 为底物。SB 咪唑预处理允许回收富含半纤维素的部分（34%），木质素脱除率为 91.2%。黑曲霉产生的木聚糖酶达到 120 小时时的 53.1 U·mL。所生产的木聚糖酶在提取木聚糖的酶解中的应用，允许生产 6.06 g·L 的 XOs，其中木三糖占>70%。对于在可持续的封闭循环中实施混合工艺以生产生物分子，同时对 SB 链的副产物进行增值，具有广阔的前景。
