Department of Pharmacology, L. M. College of Pharmacy, Gujarat Technological University, Ahmedabad, Gujarat, India.
J Basic Clin Physiol Pharmacol. 2021 Apr 23;33(4):445-455. doi: 10.1515/jbcpp-2020-0200.
Currently, there are several animal models for vasculitis. Ovalbumin and lipopolysaccharide (OVA, LPS) are well established for causing inflammation and used as an adjunct in the vasculitis induction. However, to date, none has established the effect of OVA and LPS in disease induction. Therefore, in the present study, an attempt has been made to develop a new animal model for vasculitis using OVA/LPS in rats.
A total of 42 Wistar rats were divided randomly into seven groups (n=6/group), normal control, and three different doses (0.5, 1, and 5 mg/kg) of OVA and LPS treated groups. Half of the rats in each group received only intraperitoneal sensitization, while the remaining half rats were additionally subjected to a one-week intranasal challenge.
Results showed that both OVA/LPS in their respective groups have significantly increased circulating inflammatory cells, C-reactive protein (CRP), Inflammatory cytokines (IL-1β, IL-6, TNF-α), Kidney damage markers (BUN, Creatinine), and liver function enzymes (AST, ALT) in a dose-dependent manner.
OVA/LPS induced vascular inflammation in a dose-dependent manner. However, the higher (5 mg/kg) dose of ovalbumin and lipopolysaccharide has contributed to severe vascular inflammation through increasing inflammatory cytokines. These findings suggest that OVA/LPS may contribute as a possible model for vasculitis in rats.
目前有几种血管炎动物模型。卵清蛋白和脂多糖（OVA、LPS）已被广泛用于引起炎症，并作为血管炎诱导的辅助剂。然而，迄今为止，尚无研究证实 OVA 和 LPS 在疾病诱导中的作用。因此，本研究试图使用 OVA/LPS 在大鼠中建立一种新的血管炎动物模型。
将 42 只 Wistar 大鼠随机分为 7 组（每组 6 只），正常对照组和 OVA/LPS 处理的三个不同剂量（0.5、1 和 5 mg/kg）组。每组的一半大鼠仅接受腹腔内致敏，而另一半大鼠则另外接受一周的鼻腔内挑战。
结果表明，OVA/LPS 在各自的组中均以剂量依赖性方式显著增加循环炎症细胞、C 反应蛋白（CRP）、炎症细胞因子（IL-1β、IL-6、TNF-α）、肾脏损伤标志物（BUN、肌酐）和肝功能酶（AST、ALT）。
OVA/LPS 以剂量依赖性方式诱导血管炎症。然而，较高（5mg/kg）剂量的卵清蛋白和脂多糖通过增加炎症细胞因子导致严重的血管炎症。这些发现表明，OVA/LPS 可能作为大鼠血管炎的一种可能模型。
