Department of Dermatology, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
Department of Dermatology, Longchang People's Hospital, Neijiang, China.
Skin Res Technol. 2024 Sep;30(9):e13921. doi: 10.1111/srt.13921.
Investigate Proanthocyanidins (PCs) efficacy and mechanisms in treating Henoch-Schönlein purpura (HSP)-like rat models, focusing on inflammatory and oxidative stress (OS) responses.
An HSP-like rat model was established using ovalbumin (OVA) injection, leading to symptoms mimicking HSP. The study measured inflammatory markers (IL-4, IL-17, TNF-α), OS markers (MDA, SOD, CAT), and assessed the TLR4/MyD88/NF-κB signaling pathway's involvement via histopathological and immunofluorescence analyses.
PCs treatment significantly improved HSP-like symptoms, reduced inflammatory cell infiltration, and decreased IgA deposition in renal mesangial areas. Serum analyses revealed that PCs effectively lowered IL-4, IL-17, TNF-α, and MDA levels while increasing SOD and CAT levels (p < 0.05). Crucially, PCs also downregulated TLR4, MyD88, and NF-κB expressions, highlighting the blockage of the TLR4-mediated signaling pathway as a key mechanism.
PCs show promising therapeutic effects in HSP-like rats by mitigating inflammatory responses and oxidative damage, primarily through inhibiting the TLR4/MyD88/NF-κB pathway. These findings suggest PCs as a potential treatment avenue for HSP, warranting further investigation.
研究原花青素（PCs）在治疗过敏性紫癜（HSP）样大鼠模型中的疗效和机制，重点关注炎症和氧化应激（OS）反应。
采用卵清蛋白（OVA）注射建立 HSP 样大鼠模型，模拟 HSP 症状。研究测量了炎症标志物（IL-4、IL-17、TNF-α）、OS 标志物（MDA、SOD、CAT），并通过组织病理学和免疫荧光分析评估 TLR4/MyD88/NF-κB 信号通路的参与情况。
PCs 治疗显著改善了 HSP 样症状，减少了炎症细胞浸润，降低了肾小球系膜区 IgA 沉积。血清分析显示，PCs 有效降低了 IL-4、IL-17、TNF-α 和 MDA 水平，同时提高了 SOD 和 CAT 水平（p<0.05）。重要的是，PCs 还下调了 TLR4、MyD88 和 NF-κB 的表达，突出了 TLR4 介导的信号通路的阻断是其主要机制之一。
PCs 通过减轻炎症反应和氧化损伤，对 HSP 样大鼠表现出有希望的治疗效果，主要是通过抑制 TLR4/MyD88/NF-κB 通路。这些发现表明 PCs 可能是 HSP 的一种潜在治疗方法，值得进一步研究。
