Dou Dou, Li Xiao-Kou, Xia Qi-Sheng, Chen Ying-Ying, Li Yuan-Liang, Wang Chao, Qi Zhi-Rong, Tan Huang-Ying
Beijing University of Chinese Medicine, Beijing, China.
Department of Integrative Oncology, China-Japan Friendship Hospital, Beijing, China.
BMC Gastroenterol. 2021 Apr 23;21(1):188. doi: 10.1186/s12876-021-01769-7.
Currently, there are no circulating diagnostic biomarkers for gastric neuroendocrine neoplasms (g-NENs). In previous studies, we found that miRNA-202-3p is overexpressed in the tumour tissue of type 1 g-NEN. We speculated that miRNA-202-3p is also likely to be highly expressed in circulating blood.
A total of 27 patients with type 1 g-NEN and 27 age- and sex-matched control participants were enrolled in this study. The miRNA-202-3p levels in serum obtained from the participants were measured by qRT-PCR. The expression level of miRNA-202-3p in the samples was calculated by comparison with a standard curve.
The clinical characteristics of the patients were similar to those of the patient samples in previous reports. Expression of miRNA-202-3p was significantly higher in the patient group (3.84 × 10 copies/nl) than in the control group (0.635 × 10 copies/nl). The area under the ROC curve (AUC) was 0.878 (95% CI: 0.788-0.968), and the optimal cut-off point was approximately 1.12 × 10 copies/nl. The sensitivity and specificity were 88.9% and 77.8%, respectively.
This study suggests that miRNA-202-3p is potentially useful as a biomarker of type 1 g-NEN; further investigation and verification should be performed in future research.
目前，尚无用于胃神经内分泌肿瘤（g-NENs）的循环诊断生物标志物。在先前的研究中，我们发现miRNA-202-3p在1型g-NEN的肿瘤组织中过表达。我们推测miRNA-202-3p在循环血液中也可能高表达。
本研究共纳入27例1型g-NEN患者和27名年龄及性别匹配的对照参与者。通过qRT-PCR检测参与者血清中的miRNA-202-3p水平。通过与标准曲线比较计算样本中miRNA-202-3p的表达水平。
患者的临床特征与先前报道中的患者样本相似。miRNA-202-3p在患者组中的表达（3.84×10拷贝/纳升）显著高于对照组（0.635×10拷贝/纳升）。ROC曲线下面积（AUC）为0.878（95%CI：0.788 - 0.968），最佳截断点约为1.12×10拷贝/纳升。敏感性和特异性分别为88.9%和77.8%。
本研究表明，miRNA-202-3p可能作为1型g-NEN的生物标志物；未来研究应进行进一步的调查和验证。
