Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
J Surg Oncol. 2013 Aug;108(2):89-92. doi: 10.1002/jso.23358. Epub 2013 Jun 3.
Tumor-associated miRNAs have been detected in serum or plasma. We investigated whether plasma miRNA-199a-3p could be a potential circulating biomarker for early gastric cancer (EGC).
By using real-time qRT-PCR, the expression of miRNA-199a-3p were compared between these pre-operative plasmas from 30 EGC patients and 70 healthy controls, and between these pre-operative and post-operative plasmas. Further validation was on an independent set of plasmas from 50 EGC patients.
The expression of miRNA-199a-3p (47.5 ± 6.5) in plasma in EGC patients was significantly higher than that from healthy controls (13.9 ± 2.7, P < 0.001) and gastric precancerous diseases (GPD) patients (19.2 ± 2.5, P = 0.004), respectively. Furthermore, the expression levels of miRNA-199a-3p (11.8 ± 2.9, P = 0.012) in the post-operative plasmas were significantly reduced when compared to the pre-operative plasmas. With respect of clinicopathological characteristics, the expression of miRNA-199a-3p in plasma was not associated with the depth of tumor invasion. Moreover, the AUC of the expression of miRNA-199a-3p in plasma for EGC diagnosis was 0.818, which was significantly higher than that of combined tumor markers (0.556). The sensitivity, specificity and accuracy of miRNA-199a-3p expression in plasma for EGC diagnosis were 76%, 74%, and 75%, respectively.
Plasma miRNA-199a-3p could be a novel potential diagnostic biomarkers for EGC.
肿瘤相关的 microRNA 已在血清或血浆中被检测到。我们研究了血浆 microRNA-199a-3p 是否可以作为早期胃癌（EGC）的潜在循环生物标志物。
通过实时 qRT-PCR，比较了 30 例 EGC 患者术前血浆与 70 例健康对照者、术前与术后血浆中 microRNA-199a-3p 的表达。在另外 50 例 EGC 患者的独立血浆样本中进行了进一步验证。
EGC 患者血浆中 microRNA-199a-3p 的表达（47.5±6.5）明显高于健康对照者（13.9±2.7，P<0.001）和胃癌前病变（GPD）患者（19.2±2.5，P=0.004）。此外，与术前血浆相比，术后血浆中 microRNA-199a-3p 的表达水平（11.8±2.9，P=0.012）显著降低。关于临床病理特征，血浆中 microRNA-199a-3p 的表达与肿瘤浸润深度无关。此外，血浆中 microRNA-199a-3p 表达对 EGC 诊断的 AUC 为 0.818，明显高于联合肿瘤标志物（0.556）。血浆中 microRNA-199a-3p 表达对 EGC 诊断的灵敏度、特异度和准确度分别为 76%、74%和 75%。
血浆 microRNA-199a-3p 可能是 EGC 的一种新型潜在诊断生物标志物。
