Department of Biomedical Science and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Republic of Korea.
Lett Appl Microbiol. 2021 Aug;73(2):168-175. doi: 10.1111/lam.13488. Epub 2021 May 5.
The susceptibility of Acinetobacter baumannii exposed to primary antibiotic can be either increased or decreased when exposed to secondary antibiotic. This study was designed to assess the relative fitness, collateral susceptibility and collateral resistance of polymyxin B- (PMB-) adapted A. baumannii to ciprofloxacin (CIP), meropenem (MER), PMB, tetracycline (TET) and tobramycin (TOB). Strains of wild-type A. baumannii KACC 12454 (AB ), wild-type A. baumannii CCARM 12088 (AB ), PMB-adapted AB , PMB-adapted AB , stabilized AB and stabilized AB were used in this study. Compared to the wild-type AB , the MICs of PMB were increased from 2 to 128 μg ml against PMB-adapted AB , while MICs of CIP, MER, TET and TOB were decreased from 2 to 1 μg ml , 16 to 1 μg ml , 16 to 2 μg ml and 64 to 16 μg ml , respectively. The PMB-adapted AB was resistant to CIP (32 μg ml ) and PMB (64 μg ml ) compared to the wild-type AB . The resistance of stabilized AB and AB to all antibiotics was lost after antibiotic-free culture in the exception of CIP and TET. The susceptibilities of wild-type, PMB-adapted and stabilized AB and AB to CIP, MER, PMB, TET and TOB were increased in the presence of β-lactamase and efflux pump inhibitors. The high levels of relative fitness were observed for stabilized AB , PMB-adapted AB and stabilized AB . The stabilized AB and PMB-adapted AB were highly heteroresistance to PMB and TET, respectively. The PMB-adapted AB and AB showed various antibiotic patterns, known as collateral susceptibility and collateral resistance. The results provide useful information for designing effective antibiotic regimens that can enhance the antibiotic activity against A. baumannii infections.
当接触次级抗生素时，鲍曼不动杆菌暴露于初级抗生素后的易感性可能会增加或降低。本研究旨在评估多粘菌素 B（PMB）适应的鲍曼不动杆菌对环丙沙星（CIP）、美罗培南（MER）、PMB、四环素（TET）和妥布霉素（TOB）的相对适应性、交叉易感性和交叉耐药性。本研究使用了野生型鲍曼不动杆菌 KACC 12454（AB）、野生型鲍曼不动杆菌 CCARM 12088（AB）、PMB 适应的 AB、PMB 适应的 AB、稳定的 AB 和稳定的 AB 菌株。与野生型 AB 相比，PMB 适应的 AB 对 PMB 的 MIC 从 2 增加到 128μg/ml，而 CIP、MER、TET 和 TOB 的 MIC 从 2 降低到 1μg/ml、16 降低到 1μg/ml、16 降低到 2μg/ml 和 64 降低到 16μg/ml。PMB 适应的 AB 对 CIP（32μg/ml）和 PMB（64μg/ml）的耐药性高于野生型 AB。在无抗生素培养后，除 CIP 和 TET 外，稳定的 AB 和 AB 对所有抗生素的耐药性均丧失。在β-内酰胺酶和外排泵抑制剂存在的情况下，野生型、PMB 适应的和稳定的 AB 和 AB 对 CIP、MER、PMB、TET 和 TOB 的敏感性增加。稳定的 AB、PMB 适应的 AB 和稳定的 AB 的相对适应性较高。稳定的 AB 和 PMB 适应的 AB 对 PMB 和 TET 分别表现出高度的异质性耐药性。PMB 适应的 AB 和 AB 表现出各种抗生素模式，称为交叉易感性和交叉耐药性。这些结果为设计有效的抗生素方案提供了有用的信息，这些方案可以增强抗生素对鲍曼不动杆菌感染的活性。
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