Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia.
N. I. Pirogov Russian National Research Medical University, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2021 Apr;170(6):748-751. doi: 10.1007/s10517-021-05147-x. Epub 2021 Apr 24.
We analyzed functional status of blood leukocytes in diabetes mellitus and after addition of glucose in vitro. To this end, generation of ROS and reactive halogen species by monocytes and neutrophils from patients with diabetes mellitus and healthy donors was assayed using lucigenin- and luminol-dependent chemiluminescence after stimulation with phorbol 12-myristate 13-acetate or opsonized zymosan in vitro. Formation of neutrophil extracellular traps was evaluated in the blood after addition of glucose. In comparison with donors, leukocytes from patients with diabetes mellitus were primed and this effect can be modeled by addition of glucose to the blood in vitro. Addition of glucose to donor blood also triggered the formation of neutrophil extracellular traps.
我们分析了糖尿病患者血液白细胞的功能状态以及体外添加葡萄糖后的状态。为此，我们使用荧光素和鲁米诺依赖性化学发光法，检测了糖尿病患者和健康供体的单核细胞和中性粒细胞在佛波醇 12-肉豆蔻酸 13-乙酸或调理酵母聚糖刺激后产生 ROS 和活性卤素物种的情况。在体外加入葡萄糖后，评估了血液中中性粒细胞细胞外陷阱的形成。与供体相比，糖尿病患者的白细胞被预先激活，这种效应可以通过体外向血液中添加葡萄糖来模拟。向供体血液中添加葡萄糖也会触发中性粒细胞细胞外陷阱的形成。
