Yu. M. Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical-Biological Agency of Russia, Moscow, Russia.
Bull Exp Biol Med. 2023 Dec;176(2):137-142. doi: 10.1007/s10517-024-05983-7. Epub 2024 Jan 8.
NETosis, i.e., the formation of neutrophil extracellular traps (NET), and neutrophil autophagy are important elements in the pathogenesis and the development of complications of type 2 diabetes mellitus (T2DM). Therefore, the search of drugs that can regulate the level of NETosis and autophagy in T2DM is relevant. Here we studied an ex vivo NET formation and neutrophil death in whole blood from healthy subjects upon the addition of glucose up to a high concentration of 15 mM or/and the phorbol ester PMA (phorbol-12-myristate-13-acetate). Their individual and combined action caused neutrophil death and an increase in NET content. It can be hypothesized that this resulted from activation of NETosis and autophagy. It was also shown that this activation of NETosis and autophagy is completely prevented by daily intake of 1000 IU vitamin D3 for 14 days. Therefore, vitamin D3 supplementation can be considered as a preventive measure against the development of T2DM complications.
NETosis，即中性粒细胞细胞外陷阱（NET）的形成和中性粒细胞自噬，是 2 型糖尿病（T2DM）发病机制和并发症发展的重要因素。因此，寻找能够调节 T2DM 中 NETosis 和自噬水平的药物是相关的。在这里，我们研究了健康受试者全血中 NET 形成和中性粒细胞死亡的情况，在加入葡萄糖至 15mM 或/和佛波酯 PMA（佛波醇 12-肉豆蔻酸 13-乙酸酯）的高浓度时。它们的单独和联合作用导致中性粒细胞死亡和 NET 含量增加。可以假设这是由于 NETosis 和自噬的激活。还表明，这种 NETosis 和自噬的激活完全可以通过每天摄入 1000IU 维生素 D3 持续 14 天来预防。因此，维生素 D3 的补充可以被认为是预防 T2DM 并发症发展的一种措施。
