Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russia.
Bull Exp Biol Med. 2021 Apr;170(6):778-781. doi: 10.1007/s10517-021-05153-z. Epub 2021 Apr 24.
We studied the expression of arginase-1 (Arg1) and tyrosine kinase Mer (MerTK) in GMCSF-differentiated human macrophage populations М0, М1(IFNγ), М2а(IL-4), and М2(low serum) generated under conditions of growth/serum factor deficiency. The maximum relative content of Arg1+ and MerTK+ cells was found in М2 macrophage populations: М2а(IL-4) and М2(low serum). As the uptake of apoptotic cells is the key mechanism of M2 polarization during M2(low serum) generation, we performed a special series of experiments and showed that incubation with allogeneic apoptotic neutrophils significantly increased the percentages of CD206+ macrophages co-expressing Arg1 and MerTK.
我们研究了 GMCSF 分化的人巨噬细胞群体 М0、М1（IFNγ）、М2а（IL-4）和 М2（低血清）中精氨酸酶-1（Arg1）和酪氨酸激酶 Mer（MerTK）的表达，这些群体是在生长/血清因子缺乏的条件下生成的。Arg1+ 和 MerTK+ 细胞的最大相对含量存在于 М2 巨噬细胞群体中：М2а（IL-4）和 М2（低血清）。由于摄取凋亡细胞是 M2（低血清）生成过程中 M2 极化的关键机制，我们进行了一系列特殊的实验，并表明与同种异体凋亡中性粒细胞孵育可显著增加共表达 Arg1 和 MerTK 的 CD206+ 巨噬细胞的百分比。
