Zelnickova Petra, Matiasovic Jan, Pavlova Barbora, Kudlackova Hana, Kovaru Frantisek, Faldyna Martin
Department of Immunology, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Nitric Oxide. 2008 Aug;19(1):36-41. doi: 10.1016/j.niox.2008.04.001. Epub 2008 Apr 14.
The aim of this work was to compare in vitro nitric oxide (NO) production by rat, bovine and porcine macrophages. NO production was induced by lipopolysaccharide (LPS) or by phorbol 12-myristate 13-acetate (PMA) with ionomycin or recombinant interferon gamma (rIFN-gamma) and was assessed by Griess reaction. NO synthase type II (NOS II) expression was quantified by immunocytochemistry, Western blot and real-time polymerase chain reaction (RT-PCR). There were differences in NO production by pulmonary alveolar macrophages (PAM) in all species tested. The largest amounts of NO were produced by rat PAM. Less NO was produced by bovine PAM. Moreover, PAM in rats and cows differed in their abilities to respond to various stimulators. Neither porcine PAM nor Kupffer cells produced NO. Stimulation of porcine PAM with alternative concentrations of LPS did not lead to inducing NO production. Stimulation of porcine PAM with rIFN-gamma together with LPS led to a significant increase in the expression of NOS II mRNA, albeit without detectable NO production or NOS II expression on the protein level.
这项工作的目的是比较大鼠、牛和猪巨噬细胞体外一氧化氮（NO）的产生情况。通过脂多糖（LPS）或佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）与离子霉素或重组干扰素γ（rIFN - γ）诱导产生NO，并通过格里斯反应进行评估。通过免疫细胞化学、蛋白质印迹和实时聚合酶链反应（RT - PCR）对II型一氧化氮合酶（NOS II）的表达进行定量。在所测试的所有物种中，肺泡巨噬细胞（PAM）产生NO的情况存在差异。大鼠PAM产生的NO量最多。牛PAM产生的NO较少。此外，大鼠和牛的PAM对各种刺激物的反应能力也不同。猪PAM和库普弗细胞均不产生NO。用不同浓度的LPS刺激猪PAM不会诱导产生NO。用rIFN - γ与LPS共同刺激猪PAM会导致NOS II mRNA表达显著增加，尽管在蛋白质水平上未检测到NO产生或NOS II表达。
