Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
Parasitol Int. 2021 Aug;83:102361. doi: 10.1016/j.parint.2021.102361. Epub 2021 Apr 21.
We investigated here, the prevalence of Nosema microsporidia infections in the honeybees, Apis cerana japonica and Apis mellifera, in the Tohoku region of Japan. We detected Nosema ceranae DNA in 14 (2.8%) of 509 A. cerana japonica and in 34 (21.9%) of 155 A. mellifera honeybees from Aomori, Iwate, Akita, Yamagata, and Fukushima prefectures. Nosema apis DNA was undetectable in A. cerana japonica and A. mellifera. The unidentifiable Nosema species that genetically differed from N. apis, N. ceranae, and N. neumanni in terms of small subunit (SSU) rDNA, large subunit rDNA, and internal transcribed spacer sequences was identified in 105 (20.6%) of 509 A. cerana japonica and in 1 (0.6%) of 155 A. mellifera honeybees, and from Iwate prefecture. A phylogenetic tree based on SSU rDNA sequences showed that the Nosema sp. belonged to the same clade as N. thomsoni detected in moth and solitary bees in North America and N. pieriae found in cabbage butterfly in Turkey, which have not hitherto been detected in honeybees. The morphological characteristics of the spores should be analyzed to enable species identification of the Nosema sp.
我们在这里调查了日本东北地区的日本蜜蜂（Apis cerana japonica）和西方蜜蜂（Apis mellifera）中微孢子虫 Nosema 的感染流行情况。我们在青森、岩手、秋田、山形和福岛县的 509 只 A. cerana japonica 蜜蜂中检测到了 14 只（2.8%）感染了 N. ceranae DNA，在 155 只 A. mellifera 蜜蜂中检测到了 34 只（21.9%）感染了 N. ceranae DNA。在 A. cerana japonica 和 A. mellifera 中未检测到 N. apis DNA。在 509 只 A. cerana japonica 中，有 105 只（20.6%）和 155 只 A. mellifera 蜜蜂中的 1 只（0.6%）检测到与 N. apis、N. ceranae 和 N. neumanni 在小亚基（SSU）rDNA、大亚基 rDNA 和内部转录间隔区序列上存在遗传差异的无法识别的 Nosema 物种，这些蜜蜂均来自岩手县。基于 SSU rDNA 序列构建的系统发育树显示，该 Nosema 种与在北美的蛾和独居蜜蜂中检测到的 N. thomsoni 以及在土耳其的甘蓝粉蝶中发现的 N. pieriae 属于同一分支，这些物种迄今尚未在蜜蜂中发现。应该分析孢子的形态特征，以便对该 Nosema 种进行物种鉴定。
