Chemurot Moses, De Smet Lina, Brunain Marleen, De Rycke Riet, de Graaf Dirk C
Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281 S2, B-9000 Ghent, Belgium; Department of Zoology, Entomology and Fisheries Sciences, College of Natural Sciences, Makerere University, P.O. Box 7062, Kampala, Uganda.
Laboratory of Molecular Entomology and Bee Pathology, Ghent University, Krijgslaan 281 S2, B-9000 Ghent, Belgium.
Eur J Protistol. 2017 Oct;61(Pt A):13-19. doi: 10.1016/j.ejop.2017.07.002. Epub 2017 Jul 19.
The microsporidium Nosema neumanni n. sp., a new parasite of the honeybee Apis mellifera is described based on its ultra-structural and molecular characteristics. Structures resembling microsporidian spores were found by microscopic examination of honeybees from Uganda. Molecular confirmation failed when PCR primers specific for Nosema apis and Nosema ceranae were used, but was successful with primers covering the whole family of Nosematidae. We performed transmission electron microscopy and found typical microsporidian spores which were smaller (length: 2.36±0.14μm and width: 1.78±0.06μm; n=6) and had fewer polar filament coils (10-12) when compared to those of known species infecting honeybees. The entire 16S SSU rRNA region was amplified, cloned and sequenced and was found to be unique with the highest resemblance (97% identity) to N. apis. The incidence of N. neumanni n. sp. in Ugandan honeybees was found to be much higher than of the two other Nosema species.
基于其超微结构和分子特征，描述了微孢子虫新种neumanni Nosema neumanni，它是蜜蜂Apis mellifera的一种新寄生虫。通过对来自乌干达的蜜蜂进行显微镜检查，发现了类似微孢子虫孢子的结构。当使用对apis Nosema和ceranae Nosema特异的PCR引物时，分子确认失败，但使用覆盖Nosematidae整个家族的引物时成功。我们进行了透射电子显微镜检查，发现典型的微孢子虫孢子比感染蜜蜂的已知物种的孢子更小（长度：2.36±0.14μm，宽度：1.78±0.06μm；n = 6），并且极丝盘绕较少（10 - 12个）。扩增、克隆并测序了整个16S SSU rRNA区域，发现其具有独特性，与apis Nosema的相似度最高（97%同一性）。发现neumanni Nosema neumanni在乌干达蜜蜂中的发生率远高于其他两种Nosema物种。
