Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Department of Histology and Embryology, Malá Hora 4, 036 01 Martin, Slovakia.
Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Department of Histology and Embryology, Malá Hora 4, 036 01 Martin, Slovakia.
Ann Diagn Pathol. 2021 Jun;52:151731. doi: 10.1016/j.anndiagpath.2021.151731. Epub 2021 Mar 15.
In this study we evaluated the expression of SATB2 protein in colorectal cancer (CRC) and its association with microsatellite instability (MSI) status, inflammation and hypoxia. Immunohistochemical SATB2 expression was observed in 111 CRC samples. We assessed the correlation between SATB2 expression and clinico-morphological parameters, MSI, COX-2 and HIF-1α expression. SATB2 was noticed in 92.8% CRC. We observed nuclear staining with predominantly strong immunoreaction intensity (67.6%) and percentage of SATB-2 positive cells in more than 50% of cells (87.4%). The statistically significant associations were recorded between high SATB2 expression and low grade, negative lymph nodes and negative vascular invasion. Statistical analysis confirmed a significant correlation between SATB2 expression and microsatellite stability, tendency to correlate with COX-2 and no significant correlation with HIF-1α. SATB2 is overexpressed in CRC and its high expression is a marker of good prognosis. Moreover, SATB2 expression is significantly associated with microsatellite stability, there is tendency to correlate with pro-inflammatory COX-2 and there is no association with hypoxia.
在这项研究中，我们评估了 SATB2 蛋白在结直肠癌（CRC）中的表达及其与微卫星不稳定性（MSI）状态、炎症和缺氧的关系。我们观察了 111 例 CRC 样本中 SATB2 的免疫组织化学表达。我们评估了 SATB2 表达与临床形态学参数、MSI、COX-2 和 HIF-1α表达之间的相关性。在 92.8%的 CRC 中观察到 SATB2 的核染色，主要表现为强免疫反应强度（67.6%）和阳性细胞比例超过 50%（87.4%）。高 SATB2 表达与低分级、阴性淋巴结和阴性血管浸润之间存在统计学显著相关性。统计分析证实 SATB2 表达与微卫星稳定性之间存在显著相关性，与 COX-2 呈相关性趋势，与 HIF-1α无显著相关性。SATB2 在 CRC 中过表达，其高表达是预后良好的标志物。此外，SATB2 表达与微卫星稳定性显著相关，与促炎 COX-2 呈相关性趋势，与缺氧无关。
