Kowalczyk Anna Ewa, Śliwińska-Jewsiewicka Agnieszka, Kraziński Bartłomiej Emil, Piotrowska Aleksandra, Grzegrzółka Jędrzej, Godlewski Janusz, Dzięgiel Piotr, Kmieć Zbigniew
Department of Anatomy and Histology, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-082 Olsztyn, Poland.
Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
Int J Mol Sci. 2025 Mar 6;26(5):2374. doi: 10.3390/ijms26052374.
Special AT-rich sequence-binding protein 2 (SATB2), as a nuclear matrix-associated protein and transcription factor engaged in chromatin remodeling and the regulation of gene expression, plays an important role in growth and development processes. SATB2 has been shown to have tissue-specific expression, also related to some cancers, including colorectal cancer (CRC). The aim of this study was to compare gene expression in tumor and matched non-involved colorectal tissues obtained from CRC patients, and to investigate its association with clinicopathological and demographic parameters, as well as patients' overall survival. mRNA levels in the tested tissues were assessed by quantitative polymerase chain reaction, while SATB2 protein expression was determined by immunohistochemistry. We found that the average levels of both mRNA and protein were significantly lower in tumor specimens than in matched non-involved colon tissues. Moreover, SATB2 immunoreactivity was associated with patients' sex, tumor localization, and grade of differentiation. Lower immunoreactivity of SATB2 protein was noted in high-grade tumors, in women, and in tumors located in the cecum, ascending, and transverse colon. However, the results of the present study did not show an association between expression levels and patients' overall survival. Our findings indicate the involvement of impaired SATB2 expression, significantly reduced in high-grading tumors, in the pathogenesis of CRC, while its sex- and localization-specificity should be further elucidated.
富含AT序列结合蛋白2（SATB2）作为一种与核基质相关的蛋白和参与染色质重塑及基因表达调控的转录因子，在生长和发育过程中发挥着重要作用。SATB2已被证明具有组织特异性表达，也与包括结直肠癌（CRC）在内的一些癌症相关。本研究的目的是比较从CRC患者获得的肿瘤组织和配对的未受累结直肠组织中的基因表达，并研究其与临床病理和人口统计学参数以及患者总生存期的关联。通过定量聚合酶链反应评估受试组织中的mRNA水平，而通过免疫组织化学测定SATB2蛋白表达。我们发现，肿瘤标本中mRNA和蛋白的平均水平均显著低于配对的未受累结肠组织。此外，SATB2免疫反应性与患者的性别、肿瘤定位和分化程度相关。在高级别肿瘤、女性以及位于盲肠、升结肠和横结肠的肿瘤中，SATB2蛋白的免疫反应性较低。然而，本研究结果未显示表达水平与患者总生存期之间存在关联。我们的研究结果表明，SATB2表达受损（在高级别肿瘤中显著降低）参与了CRC的发病机制，而其性别和定位特异性应进一步阐明。
