Li Chao, Li Lei, Feng Zhiyuan, Guan Lijun, Lu Fuping, Qin Hui-Min
Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, National Engineering Laboratory for Industrial Enzymes, Tianjin 300457, PR China.
Institute of Food Processing, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
Food Chem. 2021 Apr 5;357:129746. doi: 10.1016/j.foodchem.2021.129746.
d-Allulose, a low-calorie rare sugar with potential as sucrose substitute for diabetics, can be produced using d-allulose 3-epimerase (DAE). Here, we characterized a putative thermostable DAE from Pirellula sp. SH-Sr6A (PsDAE), with a half-life of 6 h at 60 °C. Bioconversion of 500 g/L d-fructose using immobilized PsDAE on epoxy support yielded 152.7 g/L d-allulose, which maintained 80% of the initial activity after 11 reuse cycles. A multienzyme cascade system was developed to convert sucrose to d-allulose comprising sucrose invertase, d-glucose isomerase and PsDAE. Fruit juices were treated using this system to convert the high-calorie sugars, such as sucrose, d-glucose, and d-fructose, into d-allulose. The content of d-allulose among total monosaccharides in the treated fruit juice remained between 16 and 19% during 15 reaction cycles. This study provides an efficient strategy for the development of functional fruit juices containing d-allulose for diabetics and other special consumer categories.
阿洛酮糖是一种低热量的稀有糖，有潜力作为糖尿病患者的蔗糖替代品，可通过阿洛酮糖3-表异构酶（DAE）来生产。在此，我们对来自皮氏藻属SH-Sr6A菌株（PsDAE）的一种假定的耐热DAE进行了表征，其在60℃下的半衰期为6小时。使用固定在环氧载体上的PsDAE对500 g/L的d-果糖进行生物转化，得到了152.7 g/L的阿洛酮糖，在11次重复使用循环后仍保持初始活性的80%。开发了一种多酶级联系统，将蔗糖转化为阿洛酮糖，该系统包括蔗糖转化酶、d-葡萄糖异构酶和PsDAE。使用该系统处理果汁，将高热量的糖，如蔗糖、d-葡萄糖和d-果糖，转化为阿洛酮糖。在15个反应循环中，处理后的果汁中总单糖中的阿洛酮糖含量保持在16%至19%之间。本研究为开发含有阿洛酮糖的功能性果汁提供了一种有效的策略，适用于糖尿病患者和其他特殊消费群体。
