Largillière C, Mélancon S B
Centre de Recherche Pédiatrique, Hopital Sainte Justine, Montréal, Quebec, Canada.
Anal Biochem. 1988 Apr;170(1):123-6. doi: 10.1016/0003-2697(88)90098-x.
A high-performance liquid chromatographic method was developed for quantification of malondialdehyde (MDA) in human plasma. Deproteinized samples were injected onto a Waters carbohydrate analysis column which was eluted with 20% (v/v) 0.03 M Tris buffer, pH 7.4, in acetonitrile. Peak absorbancy was measured at 267 nm. In contrast to data already published, we did not detect any free MDA in normal human plasma. This suggests that the classical thiobarbituric acid test is not suitable for the determination of MDA in human plasma.
开发了一种高效液相色谱法用于定量测定人血浆中的丙二醛（MDA）。将去蛋白的样品注入Waters碳水化合物分析柱，该柱用20%（v/v）0.03 M Tris缓冲液（pH 7.4）与乙腈的混合液洗脱。在267 nm处测量峰吸光度。与已发表的数据相反，我们在正常人血浆中未检测到任何游离的MDA。这表明经典的硫代巴比妥酸试验不适用于测定人血浆中的MDA。
