Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.
Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Sep 5;258:119808. doi: 10.1016/j.saa.2021.119808. Epub 2021 Apr 8.
Diseases caused by metabolic abnormalities, such as inflammation and fatty liver, which are characterized by high viscosity, so it is necessary to detect the change of viscosity in vivo and in vitro. Due to the advantages of high sensitivity, noninvasive detection, high selectivity and real-time imaging, fluorescence imaging has become an effective means to detect biological parameters of biomolecules and life systems. Therefore, we have prepared a red emitting fluorescent probe NBI-V with easy synthesis which can ensure that the probe can be developed for the widely used to detection of viscosity changes in vivo and in vitro. The probe NBI-V has good stability, high response times, selectivity, and good biocompatibility. As the viscosity of a water-glycerol system increased from 1.29 cp to 937.48 cp, the fluorescence of NBI-V was increased by about 77 times. Biological experiments showed that the probe NBI-V can target mitochondria, and the Pearson correlation coefficient was as high as 0.89. What's more, it can distinguish normal liver from fatty liver, and can detect the viscosity changes caused by inflammation in mice.
由代谢异常引起的疾病，如炎症和脂肪肝，其特征是高粘度，因此有必要在体内和体外检测粘度的变化。由于荧光成像具有灵敏度高、非侵入性检测、高选择性和实时成像等优点，已成为检测生物分子和生命系统生物参数的有效手段。因此，我们制备了一种红色发射荧光探针 NBI-V，该探针具有合成简单的优点，可以确保探针能够广泛用于体内和体外粘度变化的检测。探针 NBI-V 具有良好的稳定性、高响应时间、选择性和良好的生物相容性。随着水-甘油体系粘度从 1.29 cp 增加到 937.48 cp，NBI-V 的荧光增强了约 77 倍。生物实验表明，探针 NBI-V 可以靶向线粒体，皮尔逊相关系数高达 0.89。更重要的是，它可以区分正常肝脏和脂肪肝，并可以检测小鼠炎症引起的粘度变化。
