College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China; Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5;302:123132. doi: 10.1016/j.saa.2023.123132. Epub 2023 Jul 13.
Mitochondrial viscosity is a critical factor affecting numerous physiological processes, including phagocytosis. Abnormal viscosity in mitochondria is related to some pathological activities and diseases. Evaluating and detecting the changes in mitochondrial viscosity in vivo is crucial. Thus, a mitochondria-targeted red-emitting fluorescent probe (VP) was prepared, and can be used to detect viscosity with high selectivity and sensitivity. The synthesis of probe VP was as simple as two steps and the cost was low. In addition, the fluorescence intensity (log I) exhibited an excellent relationship with viscosity (log η) in the range of 0.5 - 2.5 (R = 0.9985) in water/glycerol mixture. It is noteworthy that the probe VP displayed the highest signal-to-noise ratio (about 50-fold) for viscosity in water and glycerol system. The probe VP can visualize the mitochondrial viscosity change in living cells. More importantly, phagocytic test for BV2 cells further demonstrated that phagocytosis decreased with increased viscosity. Furthermore, VP was successfully used for monitoring the mitophagy process induced by starvation, and mitochondrial viscosity exhibited enhancement during mitophagy. The probe was a potential tool for studying viscosity and phagocytosis.
线粒体粘度是影响许多生理过程的关键因素，包括吞噬作用。线粒体粘度异常与一些病理活动和疾病有关。评估和检测体内线粒体粘度的变化至关重要。因此，我们制备了一种线粒体靶向的红色发光荧光探针（VP），可用于高选择性和高灵敏度地检测粘度。探针 VP 的合成非常简单，只需两步，成本低廉。此外，在水/甘油混合体系中，荧光强度（log I）与粘度（log η）在 0.5-2.5 范围内表现出极好的相关性（R = 0.9985）。值得注意的是，探针 VP 在水和甘油体系中对粘度的信噪比最高（约 50 倍）。探针 VP 可可视化活细胞中线粒体粘度的变化。更重要的是，BV2 细胞的吞噬试验进一步表明，随着粘度的增加，吞噬作用减少。此外，VP 成功用于监测饥饿诱导的线粒体自噬过程，线粒体粘度在自噬过程中增强。该探针是研究粘度和吞噬作用的潜在工具。
