Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2021 Mar;497(1):112-115. doi: 10.1134/S1607672921020071. Epub 2021 Apr 24.
Human CTCF (hCTCF) is a major architectural protein in mammals. In Drosophila, the CTCF homologue (dCTCF) interacts with the BTB domain of the CP190 protein, which is involved in the establishment of open chromatin and activity of insulators. Previously, it was shown that the BTB protein Kaiso interacts with hCTCF and regulates its activity. We have carried out a detailed study of the interaction between these proteins in the yeast two-hybrid assay. Surprisingly, Kaiso did not interact with hCTCF and its Drosophila homologue. On the other hand, CP190 interacted with the C-terminus of hCTCF. The results obtained demonstrate that the interaction between CTCF and CP190 proteins is highly conserved. It is likely that humans have other BTB proteins that perform the functions described for the Drosophila CP190.
人 CTCF（hCTCF）是哺乳动物中的主要结构蛋白。在果蝇中，CTCF 同源物（dCTCF）与 CP190 蛋白的 BTB 结构域相互作用，该结构域参与开放染色质的建立和绝缘子的活性。先前已经表明，BTB 蛋白 Kaiso 与 hCTCF 相互作用并调节其活性。我们在酵母双杂交测定中对这些蛋白质之间的相互作用进行了详细研究。令人惊讶的是，Kaiso 没有与 hCTCF 及其果蝇同源物相互作用。另一方面，CP190 与 hCTCF 的 C 末端相互作用。所得结果表明，CTCF 和 CP190 蛋白之间的相互作用高度保守。人类可能有其他 BTB 蛋白，它们执行果蝇 CP190 所描述的功能。
