Department of Orthopedic Surgery, the Third Affiliated Hospital, Southern Medical University, Guangzhou, China.
Department of Orthopedic Surgery, Guangzhou Liwan Orthopedics Hospital, Guangzhou, China.
Dokl Biochem Biophys. 2021 Mar;497(1):123-129. doi: 10.1134/S1607672921020174. Epub 2021 Apr 24.
This study aimed to evaluate if the 3D printed bioactive glass porous scaffolds (BGS) can improve the reconstruction of the large bone defect. A rabbit model of large bone defects was established by making a 1.0 or 1.5 cm segmental defect in the middle of the femur bone. Then a 1.0 or 1.5 cm BGS was implanted into the bone defect. X-ray imaging showed that in both 1.0 and 1.5 cm groups, the newly formed bone tissue could be observed at 4 weeks after implantation, but a strengthened ossification trend could be observed at different time points. In the 1.0 cm group, a larger number of newly formed bone tissues were observed at 4 weeks, and in the 1.5 group, more newly formed bone tissues were found at 8 weeks. Nevertheless, ossified tissue generation on the BGS mainly completed at 12 weeks after implantation in both groups. The H&E staining revealed that the 3D BGS was easily degraded to form osteoid-like material in vivo, where the neo-ossification gradually occurred from the edge to the center. Immunohistochemical analysis showed that in the 1.0 group, protein expressions of three osteogenesis-related genes- BMP, collagen I and RUNX-2-all peaked at 8 weeks, and then gradually decreased at 12 and 18 weeks. In the 1.5 group, BMP and collagen I peaked at 18 weeks.
本研究旨在评估 3D 打印生物活性玻璃多孔支架（BGS）是否可以改善大骨缺损的重建。通过在股骨中段制造 1.0 或 1.5cm 的节段性缺损，建立了大骨缺损的兔模型。然后将 1.0 或 1.5cm 的 BGS 植入骨缺损处。X 射线成像显示，在 1.0 和 1.5cm 两组中，植入后 4 周均可观察到新形成的骨组织，但在不同时间点可观察到强化的成骨趋势。在 1.0cm 组中，在 4 周时观察到更多的新形成骨组织，而在 1.5cm 组中，在 8 周时发现更多的新形成骨组织。然而，两组中骨化组织的产生主要在植入后 12 周完成。H&E 染色显示，3D BGS 在体内易降解形成类骨质样物质，新骨化从边缘逐渐向中心发生。免疫组织化学分析显示，在 1.0cm 组中，三种成骨相关基因（BMP、胶原蛋白 I 和 RUNX-2）的蛋白表达均在 8 周时达到峰值，然后在 12 周和 18 周时逐渐下降。在 1.5cm 组中，BMP 和胶原蛋白 I 在 18 周时达到峰值。
