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超分辨率显微镜中基本问题的答案。

Answers to fundamental questions in superresolution microscopy.

机构信息

Leibniz Institute of Photonic Technology, Albert-Einstein-Strasse 9, 07745 Jena, Germany.

Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University, Helmholtzweg 4, Jena, Germany.

出版信息

Philos Trans A Math Phys Eng Sci. 2021 Jun 14;379(2199):20210105. doi: 10.1098/rsta.2021.0105. Epub 2021 Apr 26.

Abstract

This article presents answers to the questions on superresolution and structured illumination microscopy (SIM) as raised in the editorial of this collection of articles (https://doi.org/10.1098/rsta.2020.0143). These answers are based on my personal views on superresolution in light microscopy, supported by reasoning. Discussed are the definition of superresolution, Abbe's resolution limit and the classification of superresolution methods into nonlinear-, prior knowledge- and near-field-based superresolution. A further focus is put on the capabilities and technical aspects of present and future SIM methods. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 1)'.

摘要

本文针对本期专题文章的社论中提出的关于超分辨率和结构光照明显微镜(SIM)的问题给出了答案(https://doi.org/10.1098/rsta.2020.0143)。这些答案是基于我对基于光的显微镜超分辨率的个人观点,并提供了推理依据。讨论了超分辨率的定义、阿贝分辨率极限以及超分辨率方法的分类,分为非线性、先验知识和近场超分辨率。进一步的重点是当前和未来 SIM 方法的功能和技术方面。本文是 Theo Murphy 会议专题“超分辨率结构光照明显微镜(第 1 部分)”的一部分。

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