National Physical Laboratory, TW11 0LW Teddington, UK.
Department of Paediatrics, Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.
Philos Trans A Math Phys Eng Sci. 2021 Jun 14;379(2199):20200143. doi: 10.1098/rsta.2020.0143. Epub 2021 Apr 26.
Structured illumination microscopy (SIM) has emerged as an essential technique for three-dimensional (3D) and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM, from bespoke hardware and software development and the use of commercial instruments to biological applications. This special issue aims to recap recent developments as well as outline future trends. In addition to SIM, we cover related topics such as complementary super-resolution microscopy techniques, computational imaging, visualization and image processing methods. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 1)'.
结构光照明显微镜（SIM）已成为三维（3D）和活细胞超分辨率成像的重要技术。然而，迄今为止，还没有专门的研讨会或期刊专刊涵盖 SIM 的各个方面，从定制的硬件和软件开发以及商业仪器的使用到生物应用。本期特刊旨在总结最近的发展，并概述未来的趋势。除了 SIM，我们还涵盖了相关主题，如互补的超分辨率显微镜技术、计算成像、可视化和图像处理方法。本文是 Theo Murphy 会议特刊“超分辨率结构光照明显微镜（第 1 部分）”的一部分。
