School of Life Sciences, Lanzhou University, Lanzhou, Gansu Province, China.
Laboratory of Clostridium, Lanzhou Institute of Biological Products, Lanzhou, Gansu Province, China.
Bioengineered. 2021 Dec;12(1):745-754. doi: 10.1080/21655979.2021.1894798.
() is a kind of obligate anaerobic gram-positive related with intestinal diseases and antibiotic treatment. In present study, the genome was studied employing met genomic technology. Genome sequencing identified LCL126 has total size of 4,301,949 bp with a 27.97% of GC content. Specifically, 4119 predicted coding genes, 188 repeat sequences, 13 prophages and 8 gene islands were detected. Additionally, gene function analysis aspect of the function annotation, effector, and virulence were concluded that total of 3367 cluster of orthologous groups of proteins genes and classified into 24 categories, while the most outstanding class was metabolic process (1533) and catalytic activity (1498). The carbohydrate-active enzymes have been detected 127 genes, pathogenicity analysis revealed that 133 reduced and 22 increased virulence (hypervirulence) genes, while 54 unaffected and 10 loss of pathogenicity genes were found. Furthermore, perform the visualization and methylation expression were revealed that the dominant types comprised m4C, m5C, and m6C with the number of 6989, 36,666, and 3534, respectively. Overall, whole genome sequence information of LCL126 was obtained and functional prediction was revealed its possible toxicological potential genes existence.
() 是一种与肠道疾病和抗生素治疗相关的专性厌氧革兰氏阳性菌。在本研究中，采用宏基因组技术对其基因组进行了研究。基因组测序表明，LCL126 的总大小为 4,301,949bp，GC 含量为 27.97%。具体来说，预测到 4119 个编码基因、188 个重复序列、13 个噬菌体和 8 个基因岛。此外，在基因功能分析方面，对功能注释、效应子和毒力进行了分析，共鉴定出 3367 个同源基因簇蛋白基因，并分为 24 类，其中最显著的类别是代谢过程（1533 个）和催化活性（1498 个）。共检测到 127 个碳水化合物活性酶基因，致病性分析显示，有 133 个减少和 22 个增加的毒力（超毒力）基因，而有 54 个未受影响和 10 个失去致病性的基因。此外，进行可视化和甲基化表达分析表明，主要类型包括 m4C、m5C 和 m6C，数量分别为 6989、36666 和 3534。总的来说，获得了 LCL126 的全基因组序列信息，并揭示了其可能存在的毒性潜在基因的功能预测。
