Panou Aikaterini, Malousi Andigoni, Kachrimanidou Melina
Department of Microbiology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Laboratory of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
BioTech (Basel). 2025 May 31;14(2):42. doi: 10.3390/biotech14020042.
The emergence of antimicrobial resistance (AMR) in (), particularly to last-line antibiotics such as linezolid, represents a critical challenge in clinical settings. This study investigates the genomic epidemiology of linezolid-resistant , focusing on the distribution and mutational patterns of the chloramphenicol-florfenicol resistance () gene and its association with multidrug resistance. We analyzed 514 clinical isolates (354 from NCBI Pathogen Detection, 160 from EnteroBase), revealing distinct prevalence patterns among subtypes: (C) was dominant (156/354 NCBI strains; 101/160 EnteroBase strains), whereas (B) frequently harbored missense mutations (p.R247K, p.V294I, and less commonly p.A334T). The (E) subtype was exclusively identified in ribotype 027 (RT027) strains. Notably, (C) exhibited a strong association with RT017, correlating with a conserved 99 bp genomic deletion. Phylogenetic analysis linked -carriage to predominant sequence types (ST1 in NCBI strains, ST37 in EnteroBase isolates). Furthermore, the co-occurrence of with additional AMR genes conferred resistance to macrolides (erythromycin, azithromycin) and tetracyclines, indicating a convergent evolution toward multidrug resistance. These findings underscore the interplay between mutations, hypervirulent ribotypes, and AMR dissemination, necessitating enhanced surveillance to mitigate the spread of resistant lineages.
[某种细菌]中抗菌药物耐药性（AMR）的出现，尤其是对诸如利奈唑胺等最后一线抗生素的耐药性，在临床环境中构成了一项严峻挑战。本研究调查了耐利奈唑胺[某种细菌]的基因组流行病学，重点关注氯霉素-氟苯尼考耐药性（[相关基因名称]）基因的分布和突变模式及其与多重耐药性的关联。我们分析了514株临床分离株（354株来自NCBI病原体检测数据库，160株来自EnteroBase数据库），揭示了[细菌]亚型之间不同的流行模式：（C）型占主导地位（354株NCBI菌株中有156株；160株EnteroBase菌株中有101株），而（B）型经常携带错义突变（p.R247K、p.V294I，较少见的是p.A334T）。（E）型仅在核糖体分型027（RT027）菌株中被鉴定出来。值得注意的是，（C）型与RT017表现出强烈关联，这与一个保守的99 bp基因组缺失相关。系统发育分析将[相关基因]携带情况与主要序列类型联系起来（NCBI菌株中的ST1，EnteroBase分离株中的ST37）。此外，[相关基因]与其他抗菌药物耐药基因的共同出现赋予了对大环内酯类（红霉素、阿奇霉素）和四环素类的耐药性，表明朝着多重耐药性的趋同进化。这些发现强调了[相关基因]突变、高毒力核糖体分型和抗菌药物耐药性传播之间的相互作用，需要加强监测以减轻耐药[细菌]谱系的传播。
