Gao Yuan, Tang Yuhai, Gao Luyao, Niu Yingying, Gao Ruixia, Chen Xiaoyi, Hao Yi, Wang Sicen
School of Chemistry, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.
School of Chemistry, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.
Anal Chim Acta. 2021 May 29;1161:338475. doi: 10.1016/j.aca.2021.338475. Epub 2021 Apr 5.
The common elution process of molecularly imprinted polymers (MIPs) is carried out in an acidic medium, which greatly affects the stability and reusability of synthetic MIPs, especially for magnetic MIPs. In this study, we fabricated an acid-resistant imprinted layer formed by phase-transitioned lysozyme on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle, which often coexists with structural analogs. The newly designed acid-resistant imprinted layer can not only protect the internal magnetic core from denudation and dissolution, but also maintain the integrity of the imprinted layer during the elution process. The resultant magnetic MIPs exhibited good stability with no change on morphology after the repeatedly eluting process, and satisfactory reusability that can be used at least ten adsorption-desorption cycles with almost no decrease for adsorption capacity. In addition, the resultant materials possess satisfactory magnetism, uniform morphology with typical core-shell structure, stable crystallization, and good adsorption performance showing on high adsorption amount (10.82 mg g), fast kinetic equilibrium time (as short as 30 min), and satisfactory selectivity (IF = 2.85, SC > 1.5). At last, the obtained magnetic MIPs as adsorbents coupled with HPLC were successfully used to selective extract CGA in Honeysuckle samples with the high recoveries in the range of 92.0-104.4%, and the contents of CGA in Honeysuckle samples from the different origin are calculated in the range of 0.98%-1.24%.
分子印迹聚合物（MIPs）的常见洗脱过程是在酸性介质中进行的，这极大地影响了合成MIPs的稳定性和可重复使用性，尤其是对于磁性MIPs。在本研究中，我们在磁性纳米材料上制备了由相变溶菌酶形成的耐酸印迹层，用于选择性提取金银花中常与结构类似物共存的绿原酸。新设计的耐酸印迹层不仅可以保护内部磁芯不被剥蚀和溶解，还能在洗脱过程中保持印迹层的完整性。所得磁性MIPs在反复洗脱后形态无变化，表现出良好的稳定性，并且具有令人满意的可重复使用性，可用于至少十个吸附-解吸循环，吸附容量几乎没有下降。此外，所得材料具有令人满意的磁性、典型核壳结构的均匀形态、稳定的结晶以及良好的吸附性能，表现为高吸附量（10.82 mg/g）、快速的动力学平衡时间（短至30分钟）和令人满意的选择性（IF = 2.85，SC > 1.5）。最后，所制备的磁性MIPs作为吸附剂与高效液相色谱联用，成功地用于选择性提取金银花样品中的绿原酸，回收率高达92.0 - 104.4%，并计算出不同产地金银花样品中绿原酸的含量在0.98% - 1.24%范围内。
