Wang Yanhui, Yan Linlin, Zheng Guangyao
Key Laboratory of Biomass Energy and Material, Jiangsu Province, Key Laboratory of Chemical Engineering of Forest Products, National Forestry and Grassland Administration, National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China.
Plants (Basel). 2024 Dec 27;14(1):56. doi: 10.3390/plants14010056.
Rosmarinic acid (RA) is a natural active compound widely found in many plants belonging to the family of , , and so on, which has various important bioactivities, including being anti-oxidative, anti-inflammatory, antiviral, etc. Herein, novel hydrophilic magnetic molecularly imprinted polymers (HMMIPs) with a regular core-shell structure were successfully developed using RA as a template molecule, acrylamide (AM) as a functional monomer, N-N 'methylenebisacrylamide (MBA) as a cross-linking agent, and water as the porogen. After a series of characterization and adsorption performance analyses, it was found that HMMIPs are hydrophilic with an adsorption capacity of 8.012 ± 0.54 mg/g, an imprinting factor of 3.64, and a selectivity coefficient of 2.632.91. Furthermore, the HMMIPs can be rapidly separated from other components under the influence of external magnetic fields. The HMMIPs were employed for the determination of RA present in the and aqueous extract with recoveries of 88.2107.3%. These results indicated that HMMIPs of RA have the benefits of straightforward operation, rapid adsorption, and high selectivity, rendering it an appropriate way for the expedient and selective isolation of RA in an intricate matrix.
迷迭香酸（RA）是一种天然活性化合物，广泛存在于唇形科等许多植物中，具有多种重要的生物活性，包括抗氧化、抗炎、抗病毒等。在此，以RA为模板分子，丙烯酰胺（AM）为功能单体，N-N'-亚甲基双丙烯酰胺（MBA）为交联剂，水为致孔剂，成功制备了具有规则核壳结构的新型亲水性磁性分子印迹聚合物（HMMIPs）。经过一系列表征和吸附性能分析，发现HMMIPs具有亲水性，吸附容量为8.012±0.54 mg/g，印迹因子为3.64，选择性系数为2.632.91。此外，在外加磁场的作用下，HMMIPs能够快速与其他成分分离。将HMMIPs用于测定金银花和菊花水提取物中的RA，回收率为88.2107.3%。这些结果表明，RA的HMMIPs具有操作简便、吸附快速、选择性高的优点，是在复杂基质中快速、选择性分离RA的合适方法。
