Pharmaceutical Sciences Laboratory, Åbo Akademi University, FI-20520, Turku, Finland; Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520, Turku, Finland.
Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, PR China.
Anal Chim Acta. 2021 May 29;1161:338326. doi: 10.1016/j.aca.2021.338326. Epub 2021 Mar 6.
A molecularly imprinted polymer (MIP), which is synthesized by a nanomolding process around a template, has emerged as a promising analytical tool for environmental quality monitoring and food safety test. In this work, a fluoroprobe with Ag-doped MIP nanolayer (16 nm thickness) is successfully prepared for the highly selective detection of oxytetracycline (OTC) in real samples (i.e. Yangtze River water, swine urine). In the MIP nanolayer, two functional monomers (i.e. 4-(2-acrylamidoethylcarbamoyl)-3-fluorophenylboronic acid, methacrylic acid) synergistically constitute the specific recognition sites. Meanwhile, the doped Ag enhances the detection sensitivity (with a detection limit of 5.38 nM) and accelerates the detection rate (within 2.5 min) even in real samples. Therefore, the present study paves the way for the preparation of MIP-based fluoroprobes, showing great prospects in environmental quality and food safety tests.
一种分子印迹聚合物（MIP），通过在模板周围的纳米成型过程合成，已成为环境质量监测和食品安全测试的有前途的分析工具。在这项工作中，成功制备了具有掺银 MIP 纳米层（16nm 厚）的荧光探针，用于在实际样品（即长江水、猪尿）中高度选择性地检测土霉素（OTC）。在 MIP 纳米层中，两种功能单体（即 4-（2-丙烯酰胺基乙基）氨基甲酰基-3-氟苯硼酸、甲基丙烯酸）协同构成了特定的识别位点。同时，掺杂的 Ag 提高了检测灵敏度（检测限为 5.38nM），并加速了检测速度（在 2.5 分钟内），即使在实际样品中也是如此。因此，本研究为基于 MIP 的荧光探针的制备铺平了道路，在环境质量和食品安全测试中具有广阔的前景。
