Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, China; School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
Henan Key Laboratory of Biomolecular Recognition and Sensing, College of Chemistry and Chemical Engineering, Henan Joint International Research Laboratory of Chemo/Biosensing and Early Diagnosis of Major Diseases, Shangqiu Normal University, Shangqiu 476000, China.
Bioelectrochemistry. 2023 Aug;152:108450. doi: 10.1016/j.bioelechem.2023.108450. Epub 2023 Apr 25.
Silver-doped hollow carbon spheres (Ag@HCS) were firstly introduced as electrochemical probes for glycated hemoglobin (HbA) sensing at a molecularly imprinted polymer (MIP)-based carbon cloth (CC) electrode. Herein, Ag@HCS was prepared using one-pot polymerization of resorcinol and formaldehyde with AgNO on the SiO template, subsequent carbonization, and template removal. Furthermore, poly-aminophenylboronic acid (PABA) as the MIP film was used as a sensing platform for recognition of HbA, which captured the Ag@HCS probe by binding of HbA with aptamer modified on the probe surface. Due to regular geometry, large specific surface area, superior electrical conductivity, and highly-dispersed Ag, the prepared Ag@HCS probe provided an amplified electrochemical signal based on the Ag oxidation. By use of the sandwich-type electrochemical sensor, the ultrahigh sensitivity of 4.365 μA (μg mL) cm and a wide detection range of 0.8-78.4 μg mL for HbA detection with a low detection limit of 0.35 μg mL were obtained. Excellent selectivity was obtained due to the specific binding between HbA and PABA-based MIP film. The fabricated electrochemical sensing platform was also implemented successfully for the determination of HbA concentrations in the serum of healthy individuals.
首先，将掺银空心碳球（Ag@HCS）作为电化学探针，用于基于分子印迹聚合物（MIP）的碳纤维布（CC）电极上糖化血红蛋白（HbA）的传感。Ag@HCS 是通过在 SiO 模板上使用间苯二酚和甲醛与 AgNO 一锅聚合、随后碳化和模板去除来制备的。此外，聚氨苯基硼酸（PABA）作为 MIP 膜用作 HbA 的识别传感平台，其通过与探针表面修饰的适体结合来捕获 Ag@HCS 探针。由于规则的几何形状、大的比表面积、优异的导电性和高度分散的 Ag，所制备的 Ag@HCS 探针提供了基于 Ag 氧化的放大电化学信号。通过使用三明治型电化学传感器，获得了超灵敏的 4.365 μA（μg mL）cm 和宽检测范围为 0.8-78.4 μg mL，检测下限低至 0.35 μg mL。由于 HbA 和基于 PABA 的 MIP 膜之间的特异性结合，获得了优异的选择性。所构建的电化学传感平台还成功地用于测定健康个体血清中的 HbA 浓度。
