Department of Pediatrics, Southwest Hospital, Army Medical University, Chongqing, China.
Department of Thoracic Surgery, Southwest Hospital, Army Medical University, Chongqing, China.
Cancer Biomark. 2021;31(3):239-254. doi: 10.3233/CBM-200540.
The prognosis of lung cancer patients is poor without useful prognostic and diagnostic biomarker. To search for novel prognostic and diagnostic markers, we previously found homeobox-A13 (HOXA13) as a promising candidate in lung cancer.
To determine the precisely clinical feature, prognostic and diagnostic value, possible role and mechanism of HOXA13.
Gene-expression was explored by real-time quantitative-PCR, western-blot and tissue-microarray. The associations were analyzed by Chi-square test, Kaplan-Meier and Cox-regression. The roles and mechanisms were evaluated by MTS, EdU, transwell, xenograft tumor and luciferase-reporter assays.
HOXA13 expression is increased in tumors, and correlated with age of patients. HOXA13 expression is associated with unfavorable overall survival and relapse-free survival of patients in four cohorts. Interestingly, HOXA13 has different prognostic significance in adenocarcinoma (ADC) and squamous-cell carcinoma (SCC), and is a sex- and smoke-related prognostic factor only in ADC. Importantly, HOXA13 can serve as a diagnostic biomarker for lung cancer, especially for SCC. HOXA13 can promote cancer-cell proliferation, migration and invasion in vitro, and facilitate tumorigenicity and tumor metastasis in vivo. HOXA13 acts the oncogenic roles on tumor growth and metastasis by regulating P53 and Wnt/β-catenin signaling activities in lung cancer.
HOXA13 is a new prognostic and diagnostic biomarker associated with P53 and Wnt/β-catenin signaling pathways.
肺癌患者的预后较差，缺乏有用的预后和诊断生物标志物。为了寻找新的预后和诊断标志物，我们之前发现同源盒 A13（HOXA13）是肺癌有前途的候选标志物。
确定 HOXA13 的精确临床特征、预后和诊断价值、可能的作用和机制。
通过实时定量 PCR、western blot 和组织微阵列探索基因表达。通过卡方检验、Kaplan-Meier 和 Cox 回归分析相关性。通过 MTS、EdU、Transwell、异种移植肿瘤和荧光素酶报告基因检测评估作用和机制。
HOXA13 的表达在肿瘤中增加，并与患者的年龄相关。HOXA13 的表达与四个队列中患者的总生存率和无复发生存率不良相关。有趣的是，HOXA13 在腺癌（ADC）和鳞状细胞癌（SCC）中具有不同的预后意义，并且仅在 ADC 中是与性别和吸烟相关的预后因素。重要的是，HOXA13 可作为肺癌的诊断生物标志物，特别是 SCC。HOXA13 可以在体外促进癌细胞的增殖、迁移和侵袭，并促进体内肿瘤发生和肿瘤转移。HOXA13 通过调节肺癌中的 P53 和 Wnt/β-catenin 信号通路发挥致癌作用。
HOXA13 是一种与 P53 和 Wnt/β-catenin 信号通路相关的新的预后和诊断生物标志物。
