Santi Lucélia, Beys-da-Silva Walter O, Berger Markus, Yates John R, Brandelli Adriano, Vainstein Marilene H
Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul., Av. Ipiranga, 2752, suit 508, Porto Alegre, RS 90610-000 Brazil.
Hospital de Clínicas de Porto Alegre., Porto Alegre, RS Brazil.
J Food Sci Technol. 2021 May;58(5):1764-1775. doi: 10.1007/s13197-020-04688-9. Epub 2020 Aug 6.
Pectinases and other carbohydrate-active enzymes are important for the food industry, mainly for juice processing. In addition, the use of peels to produce enzymes can aggregate value to these agro-industrial residues and at the end of the process enhance qualitatively and quantitatively the juice production. In this work, three different extracts produced by LS09 using agro-industrial residues were optimized and analyzed by mass spectrometry. It was observed an increased production of pectinases in the medium containing orange peel and optimized for production of pectin lyase and pectinesterase (PE). Interestingly, not only pectinases, but also different plant cell wall degrading enzymes (i.e. glucanases, xylanases, arabinases), with a higher ratio (42/73) was identified in the medium optimized for PE. The crude extracts produced by also reveal the potential for application in the fruit juice industry, showing an increased yield and qualitative characteristics of extracted juices. The presence of other cell wall-degrading enzymes identified by proteomics, reinforce the combination for obtaining clarified and depectinized juice in a single step.
果胶酶和其他碳水化合物活性酶对食品工业很重要，主要用于果汁加工。此外，利用果皮生产酶可以提高这些农业工业残渣的价值，并在加工过程结束时在质量和数量上提高果汁产量。在这项工作中，对LS09利用农业工业残渣生产的三种不同提取物进行了优化，并通过质谱分析。观察到在含有橙皮且针对果胶裂解酶和果胶酯酶（PE）生产进行优化的培养基中，果胶酶的产量增加。有趣的是，不仅在果胶酶方面，而且在针对PE优化的培养基中还鉴定出了不同的植物细胞壁降解酶（即葡聚糖酶、木聚糖酶、阿拉伯糖苷酶），其比例更高（42/73）。同样生产的粗提取物也显示出在果汁工业中的应用潜力，提高了提取果汁的产量和质量特性。蛋白质组学鉴定出的其他细胞壁降解酶的存在，加强了一步获得澄清和脱果胶果汁的组合。
