D-半乳糖诱导断奶仔猪慢性氧化应激并改变其肠道微生物群。

D-Galactose Induces Chronic Oxidative Stress and Alters Gut Microbiota in Weaned Piglets.

作者信息

Han Hui, Liu Zemin, Yin Jie, Gao Jing, He Liuqin, Wang Chenyu, Hou Ruoxin, He Xingguo, Wang Guoqiang, Li Tiejun, Yin Yulong

机构信息

Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, China.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

出版信息

Front Physiol. 2021 Apr 8;12:634283. doi: 10.3389/fphys.2021.634283. eCollection 2021.

Oxidative stress commonly occurs in pig production, which can severely damage the intestinal function of weaned piglets. This study was conducted to investigate the effects of D-galactose with different levels used to induce chronic oxidative stress on growth performance, intestinal morphology and gut microbiota in weaned piglets. The results showed that addition of 10 and 20 g/kg BW D-galactose reduced average daily gain and average daily feed intake from the first to the third week. 10 g/kg BW D-galactose increased the concentration of serum MDA at the second and third week. 10 g/kg BW D-galactose significantly influenced the jejunal and ileal expressions of , , and . The results of 16S rRNA sequencing showed that compared with the control, 10 and 20 g/kg BW D-galactose significantly decreased the relative abundance of , , , and , while increased the relative abundance of , , and . The results indicated that treatment with 10 g/kg BW/day D-galactose for 3 weeks could induce chronic oxidative stress, reduce the growth performance and alter gut microbiota in weaned piglets.

氧化应激在养猪生产中普遍存在，会严重损害断奶仔猪的肠道功能。本研究旨在探讨不同水平的D-半乳糖诱导慢性氧化应激对断奶仔猪生长性能、肠道形态和肠道微生物群的影响。结果表明，添加10 g/kg体重和20 g/kg体重的D-半乳糖会降低第1至第3周的平均日增重和平均日采食量。10 g/kg体重的D-半乳糖会在第2周和第3周增加血清丙二醛浓度。10 g/kg体重的D-半乳糖显著影响空肠和回肠中、和的表达。16S rRNA测序结果表明，与对照组相比，10 g/kg体重和20 g/kg体重的D-半乳糖显著降低了、、和的相对丰度，同时增加了、和的相对丰度。结果表明，以10 g/kg体重/天的剂量给断奶仔猪连续3周投喂D-半乳糖可诱导慢性氧化应激，降低生长性能并改变肠道微生物群。