A Novel NAC Transcription Factor From , EgNAC141, Positively Regulates Lignin Biosynthesis and Increases Lignin Deposition.

Wood formation is a complicated process under the control of a large set of transcription factors. NAC transcription factors are considered "master switches" in this process. However, few NAC members have been cloned and characterized in , which is one of the most economically important woody plants. Here, we reported an NAC transcription factor from , EgNAC141, which has no Arabidopsis orthologs associated with xylogenesis-related processes. was predominantly expressed in lignin-rich tissues, such as the stem and xylem. Overexpression of in Arabidopsis resulted in stronger lignification, larger xylem, and higher lignin content. The expression of lignin biosynthetic genes in transgenic plants was significantly higher compared with wild-type plants. The transient expression of activated the expression of Arabidopsis lignin biosynthetic genes in a dual-luciferase assay. Overall, these results showed that EgNAC141 is a positive regulator of lignin biosynthesis and may help us understand the regulatory mechanism of wood formation.