Ingleton P M, Underwood J C, Hunt N H, Atkins D, Giles B, Coulton L A, Martin T J
Lab Anim Sci. 1977 Oct;27(5 Pt 2):748-56.
An osteogenic sarcoma was induced in an inbred strain of the Sprague Dawley rat using seven serial injections of 32P-orthophosphate. The tumor was maintained by transplantation over a 3-year period in the same inbred strain. During this time it retained its bone-like differentiation. Tumor membranes and freshly isolated tumor cells also retained responsiveness to parathyroid hormone and to prostaglandins of adenylate cyclase and cyclic nucleotide formation respectively. The potencies of these agents and their analogues and metabolites were found to be proportional to their efficacies as bone resorbing agents. Thus, the tumor was shown to be a model for the study of hormone-responsiveness for tumor growth and differentiation, and also of the effects of agonists which act on bone-like cells.
使用七次连续注射32P - 正磷酸盐在近交系斯普拉格 - 道利大鼠中诱导出骨肉瘤。该肿瘤通过在同一近交系中移植维持了3年。在此期间，它保持了骨样分化。肿瘤膜和新鲜分离的肿瘤细胞分别对甲状旁腺激素和前列腺素的腺苷酸环化酶反应以及环核苷酸形成保持反应性。发现这些药物及其类似物和代谢物的效力与它们作为骨吸收剂的功效成正比。因此，该肿瘤被证明是研究肿瘤生长和分化的激素反应性以及作用于骨样细胞的激动剂作用的模型。
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