State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P. R. China.
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
Plant Commun. 2020 Dec 15;2(2):100137. doi: 10.1016/j.xplc.2020.100137. eCollection 2021 Mar 8.
Protein-protein interaction (PPI) networks are key to nearly all aspects of cellular activity. Therefore, the identification of PPIs is important for understanding a specific biological process in an organism. Compared with conventional methods for probing PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach for characterizing PPIs. However, the application of PL remains in its infancy. Here, we summarize recent progress in PL and its potential utilization in plant biology. We specifically summarize advances in PL, including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems.
蛋白质-蛋白质相互作用 (PPI) 网络是几乎所有细胞活动的关键。因此，鉴定 PPI 对于理解生物体中的特定生物过程很重要。与传统的探测 PPI 的方法相比，最近描述的邻近标记 (PL) 方法与基于质谱 (MS) 的定量蛋白质组学相结合，已成为一种描述 PPI 的强大方法。然而，PL 的应用仍处于起步阶段。在这里，我们总结了 PL 的最新进展及其在植物生物学中的潜在应用。我们特别总结了 PL 的进展，包括不同 PL 酶的开发和比较，以及 PL 在破译不同生物体中各种分子相互作用方面的应用，重点是植物系统。
